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Integrated analysis of genome-wide profiling of lncRNA and mRNA expression and functional networks after botulinum toxin type A injection into submandibular gland of rats.

ABSTRACT: Integrated analysis of genome-wide profiling of lncRNA and mRNA expression and functional networks after botulinum toxin type A injection into submandibular gland of rats.

PROVIDER: PRJNA605627 | ENA |

REPOSITORIES: ENA

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MiRNA-mRNA expression profiles and functional network after injection of botulinum toxin type A into submandibular gland [microarray]

Project description:Backgroud: Botulinum toxin type A (BTXA) is widely used for the treatment of sialorrhea and other glandular hypersecretion diseases. Micro ribonucleic acid (miRNA) plays an important role in the occurrence and development of salivary diseases, but it is still unclear which role miRNA plays during the inhibition of salivary secretion triggered by BTXA. This work was aimed to explore the miRNA-mRNA expression profiles and functional network after injection of BTXA into submandibular gland. Methods: The differentially expressed (DE) miRNAs and mRNAs between the control group and BTXA group were identified through high-throughput sequencing and bioinformatic analyses and were confirmed by qRT-PCR. Bioinformatic analyses were performed to identify the critical biological functions and signalling pathways and established the functional network. Results: A total of 19 DE miRNAs and 1072 DE mRNAs were identified between the control group and BTXA group. We confirmed 6 DE miRNAs through the qRT-PCR validation. Bioinformatic analysis identified that several pathways may be associated with the inhibition of salivary secretion, such as MAPK signaling pathway, Tight junction and Cytokine-cytokine receptor interaction. We predicted the target genes of these 6 DE miRNAs and established the miRNA-mRNA interaction network. The intersection of DE mRNAs and target genes was performed and finally obtained 7 mRNAs: EGR2, PAQR9, ZKSCAN1, USP6N, lCYB561A3, ZFHX4 and CLIC5. Conclusions: Our study describes the miRNA and mRNA expression profiles and functional network after injection of BTXA into submandibular gland. These findings explore the mechanism of BTXA in inhibiting salivary secretion, and probably provide a new idea for the clinical application. Key words: miRNA-mRNA, BTXA, submandibular gland

2020-12-31 | GSE141819 | GEO

MiRNA-mRNA expression profiles and functional network after injection of botulinum toxin type A into submandibular gland [miRNA-Seq]

2020-12-31 | GSE141815 | GEO

MiRNA-mRNA expression profiles and functional network after injection of botulinum toxin type A into submandibular gland

Project description:MiRNA-mRNA expression profiles and functional network after injection of botulinum toxin type A into submandibular gland

| PRJNA594921 | ENA

Integrated analysis of genome-wide profiling of lncRNA and mRNA expression and functional networks after botulinum toxin type A injection into submandibular gland of rats.

Project description:Abstract: Backgroud: This study was to explore long noncoding RNA (lncRNA) and messenger RNA (mRNA) expression profiles and construct functional networks to analyze their biological functions when botulinum toxin type A (BTXA) inhibited salivary secretion. Methods: BTXA and saline were injected into the submandibular gland of rats as the BTXA group and control group, respectively. Morphological and secretory function tests validated the animal models. Microarray analysis was applied to identify the expression variation of lncRNA and mRNA and were confirmed by qRT-PCR. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were performed to explicit the biological functions. The construction of functional networks including lncRNA-mRNA co-expression network and competing endogenous RNA (ceRNA) network was to reveal the interaction between coding and noncoding genes. Results: A total of 254 lncRNAs and 631 mRNAs were differentially expressed (DE) between the control group and the BTXA group. Ten lncRNAs and eleven mRNAs were confirmed by qRT-PCR and the results were consistent with the microarray analysis. The bioinformatic analysis found that most of mRNAs were closely related to transmembrane transporter activity. LncRNA-mRNA co-expression network and ceRNA network were constructed and identified several critical mRNA-lncRNA axes and key microRNAs related to salivary secretion, respectively. Conclusions: Our study identified DE lncRNAs and mRNAs through microarray analysis and explored the biological functions and interaction between coding and noncoding genes through bioinformatics analysis. These findings may provide new directions for the mechanism of BTXA to inhibit salivary secretion.

2020-06-30 | GSE144985 | GEO

MiRNA-mRNA expression profiles and functional network after injection of botulinum toxin type A into submandibular gland [microarray]

Project description:MiRNA-mRNA expression profiles and functional network after injection of botulinum toxin type A into submandibular gland [microarray]

| PRJNA594922 | ENA

MiRNA-mRNA expression profiles and functional network after injection of botulinum toxin type A into submandibular gland [miRNA-Seq]

Project description:MiRNA-mRNA expression profiles and functional network after injection of botulinum toxin type A into submandibular gland [miRNA-Seq]

| PRJNA594908 | ENA

Genetic homogeneity among strains of Clostridium botulinum type A1 with a unique toxin gene cluster organization

2008-01-24 | GSE10223 | GEO

Genetic homogeneity among strains of Clostridium botulinum type A1 with a unique toxin gene cluster organization

Project description:Comparative genomic hybridizations were performed to compare bont/A1 strains with an A2-like toxin gene cluster organization to the genome sequenced strain, C. botulinum ATCC 3502. Keywords: comparative genomic hybridization For each hybridization, the reference strain (C. botulinum ATCC 3502) was labeled with Cy5 and each test strain was labeled with Cy3.

2010-05-26 | E-GEOD-10223 | biostudies-arrayexpress

MiRNA-mRNA expression profiles and functional network after injection of botulinum toxin type A into submandibular gland

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-12-31 | GSE141824 | GEO

Gene expression profiles for murine submandibular gland stem cells

Project description:To compare the gene expression profile of submandibular gland stem cells to submandibular gland epithelia, we have employed whole genome microarray expression profiling as a discovery platform to identify genes with the differential expression in the stem cells and the non-stem cell epithelia. Murine submandibular gland stem cells and non-stem cell epithelia are sorted through FACS

2014-07-01 | E-GEOD-46672 | biostudies-arrayexpress

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