Brushtail possums (Trichosurus vulpecula) / Stoats (Mustela erminea)
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA612791 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR11354771_1.fastq.gz | Fastqsanger.gz | |||
| SRR11354771_2.fastq.gz | Fastqsanger.gz | |||
| SRR11354772_1.fastq.gz | Fastqsanger.gz | |||
| SRR11354772_2.fastq.gz | Fastqsanger.gz | |||
| SRR11354773_1.fastq.gz | Fastqsanger.gz |
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Similar Datasets
Project description:Trichosurus vulpecula Raw sequence reads
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Project description:Partial genome characterisation of a non-cultivable marsupial adenovirus is described. Adenovirus-like particles were found by electron microscopy (EM) in the intestinal contents of brushtail possums (Trichosurus vulpecula) in New Zealand. Using degenerate PCR primers complementary to the most conserved genome regions of adenoviruses, the complete nucleotide sequence of the penton base gene, and partial nucleotide sequences of the DNA polymerase, hexon, and pVII genes were obtained. Phylogenetic analysis of the penton base gene strongly suggested that the brushtail possum adenovirus (candidate PoAdV-1) belongs to the recently proposed genus Atadenovirus. Sequence analysis of the PCR products amplified from the intestinal contents of brushtail possums originating from different geographical regions of New Zealand identified a single genotype. This is the first report of molecular confirmation of an adenovirus in a marsupial.
| S-EPMC7125522 | biostudies-literature
Project description:Trichosurus vulpecula (Brushtail possum) genome, mTriVul1, alternate haplotype
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Project description:Trichosurus vulpecula (Brushtail possum) genome, mTriVul1, primary haplotype
| PRJNA562014 | ENA
Project description:We have sequenced and characterized an endogenous type D retrovirus, which we have named TvERV(D), from the genome of an Australian marsupial, the common brushtail possum (Trichosurus vulpecula). Intact TvERV(D) gag, pro, pol, and env open reading frames were detected in the possum genome. TvERV(D) was classified as a type D retrovirus, most closely related to those of Old World monkeys, New World monkeys, and mice, based on phylogenetic analyses and genetic organization. Approximately 30 TvERV(D) proviruses are present in the genomes of possums, as detected by Southern hybridization. However, variability in fragment patterns between possums was observed and suggests recent (or ongoing) retrotranspositional activity.
| S-EPMC114837 | biostudies-literature
Project description:New Zealand suffers greatly from invasive mammal predators including rats, stoats, feral cats and possums all of which not only damage or prey on New Zealand's unique terrestrial biodiversity, but also have huge impact on NZ's economy as many of these pests act as vectors of disease to farm and game animals. As such, the NZ government has invested nearly $90 m to support an ambitious plan to make the country predator free by 2050. Although there are adequate means to control invasive predator populations, it is widely agreed that current technologies are not sufficient for total eradication and that improved technologies are required. The Achilles Heel approach is one such developmental technology that attempts to exploit variation in the genes of target species that are vital to key physiological or cellular pathways within the body, such that interference with these genes will cause a species-specific death without the harmful effects on the environment and non-targets species that the current suite of control agents engender. Interference could either be through species-specific gene knock-down using such agents as siRNA and/or the use of species-selective chemical toxicants specifically developed against these targets. To assist with identifying species-specific gene targets in the New Zealand brushtail possum (Trichosurus vulpecula) we have assembled and annotated a possum mixed heart and liver transcriptome.
| S-EPMC6831712 | biostudies-literature
Project description:Lipocalins are a family of secreted proteins. They are capable of binding small lipophilic compounds and have been extensively studied for their role in chemosignalling in rodent urine. Urine of the common brushtail possum (Trichosurus vulpecula) contains a prominent glycoprotein of 20 kDa, expressed in both sexes. We have isolated this protein and determined its primary sequence by mass spectrometry, including the use of metabolic labelling to resolve the leucine/isoleucine isobaric ambiguity. The protein sequence was identified as a lipocalin, and phylogenetic analysis grouped the protein with other marsupial lipocalin sequences in a phylogenetic clade distinct from established cross-species lipocalin sub-families. The pattern of expression in possum urine and the similarity in sequence and structure to other lipocalins suggests this protein may have a role in brushtail possum chemosignalling.
| S-EPMC7653361 | biostudies-literature
Project description:The common brushtail possum (Trichosurus vulpecula) is one of the most abundant native marsupials in urban Australia, having successfully adapted to utilize anthropogenic resources. The habituation of possums to food and shelter available in human settlements has facilitated interaction with people, pets, and zoo animals, increasing the potential for transmission of zoonotic Cryptosporidium pathogens. This study sought to examine the identity and prevalence of Cryptosporidium species occurring in possums adapted to urban settings compared to possums inhabiting remote woodlands far from urban areas and to characterize the health of the host in response to oocyst shedding. Findings indicated that both populations were shedding oocysts of the same genotype (brushtail possum 1 [BTP1]) that were genetically and morphologically distinct from zoonotic species and genotypes and most closely related to Cryptosporidium species from marsupials. The urban population was shedding an additional five Cryptosporidium isolates that were genetically distinct from BTP1 and formed a sister clade with Cryptosporidium parvum and Cryptosporidium hominis. Possums that were shedding oocysts showed no evidence of pathogenic changes, including elevated levels of white blood cells, diminished body condition (body mass divided by skeletal body length), or reduced nutritional state, suggesting a stable host-parasite relationship typical of Cryptosporidium species that are adapted to the host. Overall, Cryptosporidium occurred with a higher prevalence in possums from urban habitat (11.3%) than in possums from woodland habitat (5.6%); however, the host-specific nature of the genotypes may limit spillover infection in the urban setting. This study determined that the coexistence of possums with sympatric populations of humans, pets, and zoo animals in the urban Australian environment is unlikely to present a threat to public health safety.
| S-EPMC2546644 | biostudies-literature
Project description:Folivore marsupials, such as brushtail possum (Trichosurus Vulpecula) and koala (Phascolarctos cinereus), can metabolise higher levels of dietary terpenes, such as cineole, that are toxic to eutherian mammals. While the highly efficient drug metabolising enzymes, cytochrome P450 3A (CYP3A) and phase II conjugating enzymes (UDP-glucuronosyltransferase, UGT), are involved in the metabolism of high levels of dietary terpenes, evidence for inhibitory actions on these enzymes by these terpenes is scant. Thus, this study investigated the effect of cineole and its derivatives on catalytic activities of hepatic CYP3A and UGT in mice, rats, and possums. Results showed that cineole (up to 50 µM) and its derivatives (up to 25 µM) did not significantly inhibit CYP3A and UGT activities in mice, rats, and possums (both in silico and in vitro). Interestingly, basal hepatic CYP3A catalytic activity in the possums was ~20% lower than that in rats and mice. In contrast, possums had ~2-fold higher UGT catalytic activity when compared to mice and rats. Thus, these basal enzymatic differences may be further exploited in future pest management strategies.
| S-EPMC8698377 | biostudies-literature