Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Unique Properties of a Subset of Human Pluripotent Stem Cells With High Capacity for Self-Renewal [FAST-ATAC]

ABSTRACT: Unique Properties of a Subset of Human Pluripotent Stem Cells With High Capacity for Self-Renewal [FAST-ATAC]

PROVIDER: PRJNA613902 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Unique Properties of a Subset of Human Pluripotent Stem Cells With High Capacity for Self-Renewal [FAST-ATAC]

Project description:Archetypal human pluripotent stem cells (hPSC) are widely considered to be equivalent in developmental status to mouse epiblast stem cells, which correspond to pluripotent cells at a late post-implantation stage of embryogenesis. Heterogeneity within hPSC cultures complicates this interspecies comparison. Here we show that a subpopulation of archetypal hPSC enriched for high self-renewal capacity (ESR) has distinct properties relative to the bulk of the population, including a cell cycle with a very low G1 fraction and a metabolomic profile that reflects a combination of oxidative phosphorylation and glycolysis. ESR cells are capable of differentiation into primordial germ cell-like cells. Global DNA methylation levels in the ESR subpopulation are lower than those in mouse epiblast stem cells. Chromatin accessibility analysis revealed a unique set of open chromatin sites in ESR cells. RNA-seq at the subpopulation and single cell levels shows that, unlike mouse epiblast stem cells, the ESR subset of hPSC displays no lineage priming, and that it can be clearly distinguished from gastrulating and extraembryonic cell populations in the primate embryo. ESR hPSC correspond to an earlier stage of post-implantation development than mouse epiblast stem cells.

2020-03-30 | GSE147338 | GEO

Unique Properties of a Subset of Human Pluripotent Stem Cells With High Capacity for Self-Renewal [RNA-Seq]

2020-03-29 | GSE119324 | GEO

Unique Properties of a Subset of Human Pluripotent Stem Cells With High Capacity for Self-Renewal [scRNA-Seq]

2020-03-29 | GSE119323 | GEO

Unique Properties of a Subset of Human Pluripotent Stem Cells With High Capacity for Self-Renewal

Project description:Unique Properties of a Subset of Human Pluripotent Stem Cells With High Capacity for Self-Renewal

| PRJNA488792 | ENA

Unique properties of a subset of human pluripotent stem cells with high capacity for self-renewal.

Project description:Archetypal human pluripotent stem cells (hPSC) are widely considered to be equivalent in developmental status to mouse epiblast stem cells, which correspond to pluripotent cells at a late post-implantation stage of embryogenesis. Heterogeneity within hPSC cultures complicates this interspecies comparison. Here we show that a subpopulation of archetypal hPSC enriched for high self-renewal capacity (ESR) has distinct properties relative to the bulk of the population, including a cell cycle with a very low G1 fraction and a metabolomic profile that reflects a combination of oxidative phosphorylation and glycolysis. ESR cells are pluripotent and capable of differentiation into primordial germ cell-like cells. Global DNA methylation levels in the ESR subpopulation are lower than those in mouse epiblast stem cells. Chromatin accessibility analysis revealed a unique set of open chromatin sites in ESR cells. RNA-seq at the subpopulation and single cell levels shows that, unlike mouse epiblast stem cells, the ESR subset of hPSC displays no lineage priming, and that it can be clearly distinguished from gastrulating and extraembryonic cell populations in the primate embryo. ESR hPSC correspond to an earlier stage of post-implantation development than mouse epiblast stem cells.

| S-EPMC7229198 | biostudies-literature

Unique Properties of a Subset of Human Pluripotent Stem Cells With High Capacity for Self-Renewal [scRNA-Seq]

Project description:Unique Properties of a Subset of Human Pluripotent Stem Cells With High Capacity for Self-Renewal [scRNA-Seq]

| PRJNA488794 | ENA

Unique Properties of a Subset of Human Pluripotent Stem Cells With High Capacity for Self-Renewal [RNA-Seq]

Project description:Unique Properties of a Subset of Human Pluripotent Stem Cells With High Capacity for Self-Renewal [RNA-Seq]

| PRJNA488795 | ENA

Unique Properties of a Subset of Human Pluripotent Stem Cells With High Capacity for Self-Renewal

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-03-29 | GSE119326 | GEO

Phf6-null hematopoietic stem cells have enhanced self-renewal capacity and oncogenic potentials

Project description:Phf6-null hematopoietic stem cells have enhanced self-renewal capacity and oncogenic potentials

| PRJNA531434 | ENA

The thyroid hormone receptor β inhibits self-renewal capacity of breast cancer stem cells

Project description:The thyroid hormone receptor β inhibits self-renewal capacity of breast cancer stem cells

| PRJNA483781 | ENA

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data