Project description:We generated time-resolved profiles of fasting-induced changes in the genomic landscapes of hepatocytes and liver macrophages using the INTACT methodology

2022-01-27 | GSE147922 | GEO

Hepatocyte-Specific Genomic Profiling of the Hepatic Fasting Response

Project description:We generated profiles of fasting-induced changes in the genomic landscapes of in vivo hepatocytes using the INTACT methodology.

2021-08-30 | GSE181937 | GEO

Cell Type-Specific Transcriptional Profiling of the Hepatic Fasting Response

Project description:We generated time-resolved profiles of fasting-induced changes in the gene landscapes of hepatocytes and liver macrophages using the INTACT methodology

2022-01-27 | GSE147923 | GEO

Hepatocyte-Specific Transcriptional Profiling of the Hepatic Fasting Response

Project description:We generated transcriptional profiles of fasting-induced changes of in vivo hepatocytes using the INTACT methodology.

2021-08-30 | GSE181940 | GEO

Comparison of hepatic gene expression in response to fasting or 2 week high fat diet

Project description:The metabolic syndrome represents a cluster of well-documented risk factors for the development of type 2 diabetes and cardiovascular disease. Next to visceral obesity, dyslipidemia and insulin resistance, excessive triglyceride accumulation in the liver has been implicated to play a role in the development of the metabolic syndrome. To investigate the underlying molecular changes leading to hepatic steatosis we performed microarray analysis on livers of mice either fasted over night or fed a high fat diet for 2 Weeks. We analysed 7 500 genes and subsequently performed a pathway analysis to identify changes in hepatic genes in both models. Fasting induced a high number of differentially expressed hepatic genes, resulting in an change towards an energy saving phenotype. In contrast only a small number of genes were differentially expressed after high fat diet. Fasting promoted gluconeogenesis and b-oxidation, strongly suppressed cholesterol synthesis and activated pathways to preserve hepatic function. High fat diet induced steatosis was accompanied by the activation of the stearoyl-CoA desaturase and the lipogenic transcription factor Srebp-1c, both implicated in the development of hepatic insulin resistance. These changes reflect the activation of different gene expression programs in response to plasma lipid overload. Keywords: Diet intervention Two conditions, fasting and high fat diet. 5 biological replicates for comparison of high fat diet versus fasting and controls versus high fat diet, 4 biological replicates for the comparison of controls versus fasting. All biological replicates are performed as technical replicates in the form of a dye-swap. Total number of arrays hybridises is therefore 28.

2011-04-02 | E-GEOD-8576 | biostudies-arrayexpress

Effects of extensive fasting on hepatic transcription

2009-11-23 | GSE13064 | GEO

Effects of extensive fasting on hepatic transcription

Project description:Temporally restricted feeding has a profound effect on the hepatic circadian clock. While the circadian clock is largely unaffected by by extensive fasting, many transcripts are known to be affected by a fasting paradigm. This dataset shows the effect of extensive fasting on dynamic gene expression in the liver C57/B6 mice were entrained to ad libitum feeding schedule for two weeks. They were then released into constant were food was withdrawn at CT16. On the second day in constant darkness tissue was collected at the indicated timepoints. Total RNA was extracted and 5ug of total RNA was used for the standard Affymetrix protocol of amplification, labeling and hybridization

2009-11-23 | E-GEOD-13064 | biostudies-arrayexpress

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