Ontology highlight
ABSTRACT:
PROVIDER: PRJNA627499 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:We sequenced dissected ovaries and testes (with reproductive tracts) as well as female and male carcasses in two species of Drosophila in order to validate gene predictions from the ModENCODE project. Comparison of dissected reproductive tracts and remaining carcasses between D. simulans and D. pseudoobscura
2011-08-26 | E-GEOD-31302 | biostudies-arrayexpress
Project description:We sequenced dissected ovaries and testes (with reproductive tracts) as well as female and male carcasses in two species of Drosophila in order to validate gene predictions from the ModENCODE project.
2011-08-26 | GSE31302 | GEO
Project description:This study aimed at measuring transcriptome-wide gene expression in four populations of the copepod T. californicus. Populations were exposed to two different thermal regime, a variable 20-28 degree and a constant 20 degree followed by a one-time stress at 28 degrees. RNA was extracted at 20 and 28 degrees for all populations from all thermal regimes. Transcriptome-wide gene expression was measured, and differentially expressed genes were determined for all populations for the different temperature combinations. The results show that each populations responds to the changes in temperature in a drastically different manner (in terms of gene expression), which can have implications regarding the way these and other organisms will respond to climate change. Two southern and two northern populations of Tigriopus californicus were exposed to a variable 20-28 degree and a constant 20 degree followed by a one-time stress at 28 degrees. RNA was extracted at 20 and 28 degrees for all populations from all thermal regimes. mRNA profiles were generated by 100bp single end sequencing in the Illumina HiSeq 2000 and 2500, with one replicate per population per treatment.
2016-07-25 | E-GEOD-80737 | biostudies-arrayexpress
Project description:The formation of new species is often a consequence of genetic incompatibilities accumulated between populations during allopatric divergence. When divergent taxa interbreed, these incompatibilities impact physiology and have a direct cost resulting in reduced hybrid fitness. Recent surveys of gene regulation in interspecific hybrids have revealed anomalous expression across large proportions of the genome, with 30-70% of all genes apparently misexpressed, mostly in the direction of down-regulation. However, since most of these studies have focused on pairs of species exhibiting high degrees of reproductive isolation, the association between regulatory disruption and reduced hybrid fitness prior to species formation remains unclear. Within the copepod species Tigriopus californicus, interpopulation hybrids show reduced fitness associated with mitochondrial dysfunction. Here we show that in contrast to studies of interspecific hybrids, only 1.2% of the transcriptome was misexpressed in interpopulation hybrids of T. californicus, and nearly 80% of misexpressed genes were overexpressed rather than underexpressed. Moreover, many of the misexpressed genes were components of functional pathways impacted by mitonuclear incompatibilities in hybrid T. californicus (e.g., oxidative phosphorylation and antioxidant response). We also show that the magnitude of hybrid misregulation is not dependent on levels of protein sequence divergence, even though the latter is correlated with expression divergence between parental populations. Our results suggest that hybrid breakdown at early stages of speciation may result from initial incompatibilities amplified by the cost of compensatory physiological responses.
2015-01-05 | GSE62672 | GEO
Project description:We performed CAGE-Seq on dissected ovaries and testes as well as female and male carcasses of two species of Drosophila (D. melanogaster and D. pseudoobscura). These data are used to map transcription start sites. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf For both species, D. melanogaster and D. pseudoobscura, CAGE-Seq was performed on dissected gonads (with reproductive tracts) and remaining carcasses from wild-type adults, using Illumina GAIIx (SRA Study SRP001602, Bioproject PRJNA36315). There are eight samples. Only Drosophila melanogaster testis samples were performed in biological duplicate. See Chen ZX et al. Genome Res 2014, 24(7):1209-23.
2015-02-26 | E-GEOD-66284 | biostudies-arrayexpress
Project description:Whole transcriptome RNA sequencing in A549 human NSCLC cells was generated. A549 cells were cultured in sera isolated from male P. californicus that differed in bonding experiences
2021-03-01 | GSE167827 | GEO
Project description:Tigriopus californicus Transcriptome or Gene expression
| PRJNA304270 | ENA
Project description:Tigriopus californicus Transcriptome or Gene expression
| PRJNA308869 | ENA