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Exophiala dermatitidis

ABSTRACT: Exophiala dermatitidis population sequencing from a CF patient

PROVIDER: PRJNA628510 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	JAJGCB01.dat.gz	Other
	JAJGCB01.fasta.gz	Fasta.gz
	JAJGCB01.master.dat	Other
	JAJJBZ01.dat.gz	Other

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From glacier to sauna: RNAseq of the human pathogen Exophiala dermatitidis

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Project description:The melanized yeast Exophiala dermatitidis is resistant to many environmental stresses, and is used as a model for understanding the diverse roles of melanin in fungi. To further our understanding resistance mechanism of E. dermatitidis to acute γ-radiation exposure and whether melanin plays a role in this process, we performed RNA-seq on WT and the melanin deficient mutant Δpks1 cultures exposed to γ-radiation at the dose of 1000 Gy, which resulted in ~50% cell deaths in both strains. We observed a dramatic transcriptomic response to γ-radiation that mobilizes pathways involved in morphological development, protein degradation, and DNA repair, and is unaffected by the presence of melanin.

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The transcriptomic and phenotypic response of the melanized yeast Exophiala dermatitidis to ionizing particle exposure

Project description:The yeast E. dermatitidis is highly resistant to ionizing particle irradiation, and exposure to this stress elicits a transcriptomic response that is distinct from that induced by photonic irradiation. Melanin, in this organism, does not exhibit substantial control over this transcriptomic response, nor does it protect the cells from radiation-induced death under the conditions we tested. However, the strong response we observed here at the gene expression level inprovides insights into the unique types of damage that particle irradiation causes to fungal cells, allowing for future dissection of radiation source-specific resistance mechanisms.

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