Pterocarya stenoptera
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ABSTRACT:
PROVIDER: PRJNA634021 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Pterocarya stenoptera Transcriptome or Gene expression
| PRJNA482488 | ENA
Project description:Pterocarya stenoptera Raw sequence reads
| PRJNA589254 | ENA
Project description:Pterocarya stenoptera Raw sequence reads
| PRJNA589251 | ENA
Project description:Pterocarya stenoptera Raw sequence reads
| PRJNA639942 | ENA
Project description:Pterocarya stenoptera Raw sequence reads
| PRJNA558846 | ENA
Project description:Premise of the studyMicrosatellite markers of Pterocarya stenoptera (Juglandaceae) were developed for future studies on the population genetic diversity and spatial genetic structure of the species.Methods and resultsBased on Illumina sequencing of the transcriptome of P. stenoptera, a total of 2452 microsatellites were identified from 83,674 assembled unigenes. One hundred microsatellites were randomly selected to design amplification primer pairs. Of these, 15 were successfully amplified and displayed polymorphism. For these markers, the number of alleles per locus and population ranged from one to six. The levels of observed and expected heterozygosity varied from 0.000 to 1.000 and 0.000 to 0.718, respectively. Furthermore, all of the 15 loci were successfully cross-amplified in another congeneric species (P. hupehensis) and were demonstrated to be polymorphic.ConclusionsThe microsatellite loci described here can be used for future population genetic and landscape genetic studies on P. stenoptera.
| S-EPMC6303151 | biostudies-literature
Project description:BackgroundDrought is the main stress factor for the cultivation of Pterocarya stenoptera in urban areas, and this factor will cause its dehydration and affect its growth. Identifying drought-related genes will be useful for understanding the drought adaptation mechanism of P. stenoptera.ResultsWe used physiological indicator detection, comparative transcriptome sequencing, and reanalysis on the results of previous landscape genomics studies to investigate the drought adaptation mechanism in P. stenoptera. The changes in malondialdehyde content showed that P. stenoptera was remarkably affected by drought stress, and the increase in soluble sugar content suggested its important role in response to drought stress. Results of comparative transcriptome sequencing showed that P. stenoptera initiated a series of programs, such as increasing the gene expression of unsaturated fatty acids, tyrosine, and plant pathogen resistance, to deal with the transient drought stress. According to the annotated results in a previous study, P. stenoptera adapts to the long-term differential drought stress by regulating the thickness of cell walls and expressing upper or lower limits of the downstream genes in the hormone signaling pathway. Through the comparative analysis of drought-responsive and -adaptive genes in P. stenoptera, this study supports the hypothesis that the environment-responsive genes (ERGs) introduced by the transient environmental stresses will be substantially more than the environment-adaptive genes (EAGs) in response to long-term differential environmental stresses, and the EAGs are not necessarily ERGs.ConclusionsOur study identified drought-responsive and -adaptive genes in P. stenoptera and revealed that P. stenoptera increased the gene expression of unsaturated fatty acids, tyrosine, and plant pathogen resistance in response to transient drought stress. This study reveals the different adaptation mechanism of P. stenoptera under the transient and long-term differential drought stresses.
| S-EPMC7934232 | biostudies-literature
Project description:Pterocarya macroptera var. delavayi Raw sequence reads
| PRJNA691730 | ENA