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Single cell transcriptomic analysis of KLB expressing cells in the hypothalamus

ABSTRACT: Single cell transcriptomic analysis of KLB expressing cells in the hypothalamus

PROVIDER: PRJNA634096 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11818052_1.fastq.gz	Fastqsanger.gz
	SRR11818052_2.fastq.gz	Fastqsanger.gz
	SRR11818053_1.fastq.gz	Fastqsanger.gz
	SRR11818053_2.fastq.gz	Fastqsanger.gz
	SRR11818054_1.fastq.gz	Fastqsanger.gz

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Single cell transcriptomic analysis of KLB expressing cells in the hypothalamus

Project description:Using a custom primer set for cDNA library construction we performed single cell RNA sequencing on FACs sorted tdTomato positive cells from single cell suspensions prepared from the hypothalamus of mice expressing cre-recombinase under the endogenus KLB promoter that were crossed with tdTomato reporter mice (Ai14). We then performed unbiased clustering analysis on 1,904 cells using the single cell transcriptomes to identify 12 different cell types which express KLB in the hypothalamus including neuronal and non-neuronal cell types. This information was then used to delete KLB in specific cell populations to determine the direct target of FGF21 in the brain responsible for suppression of sucrose preference.

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Project description:Alterations in metabolism, sleep patterns, body composition, and hormone status are all key features of aging. The hypothalamus is a well-conserved brain region that controls these homeostatic and survival-related behaviors. Despite the importance of this brain region in healthy aging, little is known about the intrinsic features of hypothalamic aging. Here, we utilize single nuclei RNA-sequencing to assess the transcriptomes of 40,064 hypothalamic nuclei from young and aged female mice. We identify cell type-specific signatures of aging in neurons, astrocytes, and microglia, as well as among the diverse collection of neuronal subtypes in this region. We uncover key changes in cell types critical for metabolic regulation and body composition, as well as in an area of the hypothalamus linked to cognition. In addition, our analysis reveals female-specific changes in sex chromosome regulation in the aging brain. This study identifies critical cell-specific features of the aging hypothalamus in mammals.

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