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PROVIDER: PRJNA634435 | ENA |
REPOSITORIES: ENA
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Project description:Withering is an important biological process associated with dehydration and cell wall metabolism in postharvest plant organs during curing/processing and storage. However, the withering mechanisms involved in dehydration and cell wall metabolism in postharvest tobacco leaves remain elusive. Here, TMT-based quantitative proteomic analysis in tobacco leaves under different withering conditions was performed. In total, 496 differentially abundant proteins (DAPs) were identified. To elucidate the withering mechanism of tobacco leaves, 28 DAPs associated with cell wall metabolism were screened.
2019-08-03 | PXD014902 | iProX
Project description:Microbial community of air-cured tobacco leaves
| PRJNA276980 | ENA
Project description:Comparative analysis of tobacco leaves transcriptomes unveils carotenoid pathway potentially determined the characteristics of aroma compounds in different environmental regions. Tobacco (Nicotiana tabacum) is a sensitive crop to environmental changes, and a tobacco with unique volatile aroma fractions always formed in specific ecological conditions. In order to investigate the differential expressed genes caused by environmental changes and reveal the formation mechanism of characteristics of tobacco in three different aroma tobacco regions of Guizhou Province, Agilent tobacco microarray was adapted for transcriptome comparison of tobacco leaves in medium aroma tobacco region Kaiyang and light aroma tobacco regions Weining and Tianzhu. Results showed that there was big difference among the gene expression profiles of tobacco leaves in different environmental conditions. A total of 517 differential expressed genes (DEGs) between Weining and Tianzhu were identified, while 733 and 1,005 genes differentially expressed between Longgang and another two tobacco regions Weining and Tianzhu, respectively. Compared with Longgang, up-regulated genes in Weining and Tianzhu were likely involved in secondary metabolism pathways, especially carotenoid pathway, including PHYTOENE SYNTHASE, PHYTOENE DEHYDROGENASE, LYCOPENE ε-CYCLASE, CAROTENOID β-HYDROXYLASE and CAROTENOID CLEAVAGE DIOXYGENASE 1 genes, while most down-regulated genes played important roles in response to temperature and light radiation, such as heat shock proteins. Gene Ontology and MapMan analyses demonstrated that the DEGs among different environmental regions were significantly enriched in light reaction of photosystem II, response of stimulus and secondary metabolism, suggesting they played crucial roles in environmental adaptation and accumulation of aroma compounds in tobacco plants. Through comprehensive transcriptome comparison, we not only identified several stress response genes in tobacco leaves from different environmental regions but also highlighted the importance of carotenoid pathway genes for characteristics of aroma compounds in specific growing regions. Our study primarily laid the foundation for further understanding the molecular mechanism of environmental adaptation of tobacco plants and molecular regulation of aroma substances in tobacco leaves.
2012-02-23 | GSE36008 | GEO
Project description:Tobacco (Nicotiana tabacum L.) is an important cash crop, and the size of its leaves significantly influences both yield and quality. However, the upper part of tobacco leaves, due to its dense tissue structure, often faces issues such as narrow and thick leaves during the production of roasted cigarettes. These problems have a severe impact on the yield and quality of the upper leaf. Although the mechanism of leaf size regulation in Arabidopsis thaliana has been extensively studied, it remains unclear for tobacco. Therefore, this research aimed to investigate the role of the NtAN3 gene in regulating tobacco leaf size by utilizing the NC82 variety. The researchers created both an overexpression mutant (G27) and a silencing mutant (M21) of the NtAN3 gene and examined their impact on leaf size using cell morphology observation and transcriptome analysis. These research findings offer valuable insights for molecular breeding aimed at improving tobacco yield and enhancing the availability of upper leaves.
2023-10-31 | GSE246454 | GEO
Project description:To investigate the effect of soy peptides on gut microial composition during juvenile social isolation, group-house (GH) and social isolation (SI) mice were fed a diet consisting of soy peptides or a control diet for 4 weeks post-weaning. We then performed microbial community analysis using data obtained from bacterial 16S rRNA gene sequencing in the fecal samples of 4 mice groups (control diet-fed GH, soy peptide-diet fed GH, control diet-fed SI, and soy peptide-diet fed SI mice).
2024-05-29 | GSE231557 | GEO
Project description:Senescence (or aging) is the final stage of plant development; senescence involves a complex programmed process that is closely related to biotic and abiotic stresses and is regulated by fine-tuned molecular mechanisms. Postharvest vegetative organs undergo a series of physiological and biochemical changes during senescence. However, if postharvest plant organs were not scientifically processed or cured, there would be a great loss in their commercial value during curing or storage. Thus, studying the molecular mechanisms underlying senescence of plant organs will enhance understanding of such a fundamental biological process and might provide new insights into controlling senescence and improving commercial value during curing or storage. However, few studies have focused specifically on the molecular mechanism of leaf senescence affecting tobacco quality at the proteomic level during the yellowing stage. The main objectives of this work were to determine the protein profile changes associated with different curing stages and to establish links between protein profile changes and senescence physiology to provide a more in-depth understanding of the mechanism of senescence in postharvest tobacco leaves under oxidation, heat/high temperature, dehydration and starvation stresses at the proteomic level. This study provides new insights into the postharvest physiology and molecular mechanism of senescence at the proteomic level in postharvest leaves of tobacco, a model solanaceous plant, in response to curing and senescence during the yellowing stage.
2020-01-06 | PXD016941 | iProX