Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Cell-type-specific transcriptomic perturbations in a mouse model of HNRNPU haploinsufficiency

ABSTRACT: Cell-type-specific transcriptomic perturbations in a mouse model of HNRNPU haploinsufficiency

PROVIDER: PRJNA640180 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Cell-type-specific transcriptomic perturbations in a mouse model of HNRNPU haploinsufficiency

Project description:We obtained cell-type-specific transcriptomic profiles from the neocortex and hippocampal brain regions of a mouse model of HNRNPU haploinsufficiency.

2020-06-18 | GSE152715 | GEO

HNRNPU regulates lncRNAs nuclear localization

Project description:To elucidate the role of HNRNPU in regulation of lncRNAs nuclear localization, we knocked down HNRNPU in K562 cells using short interfering RNAs and performed subcellular RNA-seq using nuclear or cytosolic RNAs

2021-01-23 | GSE165310 | GEO

Gene expression and splicing regulation by hnRNPU in skeletal muscle

Project description:Hnrnpu is the largest member of the heterogenous nuclear ribonucleoprotein family of RNA binding proteins. Hnrnpu is involved in pre-mRNA alternative splicing regulation. We used high throughput sequencing to determine how Hnrnpu regulates skeletal muscle physiology.

2020-07-06 | GSE145712 | GEO

Evidence of shared transcriptomic dysregulation of HNRNPU-related disorder between human organoids and embryonic mice

Project description:Generating effective therapies for neurodevelopmental disorders has remained elusive. An emerging drug discovery approach for neurodevelopmental disorders is to characterize transcriptome wide dysregulation in an appropriate model system and screen therapeutics based on their capacity to restore functionally relevant expression patterns. We characterized transcriptomic dysregulation in a human model of HNRNPU-related disorder to explore the potential of such a paradigm. We identified widespread dysregulation in functionally relevant pathways and then compared dysregulation in a human model to transcriptomic differences in embryonic and perinatal mice to determine whether dysregulation in an in vitro human model is partially replicated in an in vivo model of HNRNPU-related disorder. Strikingly, we find enrichment of co-dysregulation between 45-day-old human organoids and embryonic, but not perinatal, mice from distinct models of HNRNPU-related disorder. Thus, hnRNPU deficient human organoids may only be suitable to model transcriptional dysregulation in certain cell types within a specific developmental time window.

2022-12-09 | GSE219317 | GEO

Next Generation Sequencing Analysis of control and knockdown of hnRNPU in RKO cells

Project description:We knockdown the control and the hnRNPU gene in the RKO cells, then extracted the total RNAs and performed the next generation sequencing. By comparing sequencing data from control and knockdown of hnRNPU cells,we profield the downstream targets of the hnRNPU gene.

2022-12-10 | GSE141771 | GEO

TAB2 haploinsufficiency

Project description:TAB2 haploinsufficiency

| PRJNA747894 | ENA

The Heterogeneous Nuclear Ribonucleoprotein U (HNRNPU) is the Safeguard of Neural Stem Cell Viability

Project description:To study Hnrnpu function during cortical development we preformed single-cell RNA sequencing (scRNA-seq) on mouse neutrosphere cultures derived from E13 ICR cortices, 24 hours following treatment with CRISPR/CAS9 and two sgRNA sequences targeting mouse Hnrnpu. In parallel, we analyzed bulk RNA-seq from dissected cortices of E13 embryos in which Hnrnpu conditional truncation in the telencephalon was driven by Emx1::Cre. Additionally, we analyzed MARS-Seq trancriptomal profiles of cortices of E13 embryos carrying homozygous heterozygous or wildtype alleles of Hnrnpu truncation (Emx1::Cre driver) in combination with homozygous or heterozygous conditional deletion of Tp53

2022-06-07 | GSE181527 | GEO

Genome-wide maps of nuclear lamina interactions in AML12 cells upon HNRNPU KD [DamID]

Project description:Higher-order chromatin conformation plays critical role in regulating gene expression and biological development, here we show that HNRNPU, a nuclear matrix attachment factor, is a regulator of 3D genome architecture at multiple levels in mouse hepatocytes. We demonstrate that depletion of HNRNPU results into a global reorganization of nuclear bodies and re-localization of chromatin towards nuclear periphery. Additionally, upon HNRNPU depletion, chromatin interactions between A-type (active) and B-type (inactive) compartments increase significantly but those among same types of compartments decrease significantly, which associate with global gene expression changes. While TADs remain largely invariant, both inter- and intra-TAD interactions increase significantly in A-type compartments but decrease in B-type compartments. Mechanically, HNRNPU complexes with structural proteins CTCF and RAD21; depletion of HNRNPU specifically weakens the bindings of RAD21 to the chromatin, which is highly correlated with the weakness of chromatin loops.

2017-12-03 | GSE95115 | GEO

HNRNPU regulates lncRNAs nuclear localization

Project description:HNRNPU regulates lncRNAs nuclear localization

| PRJNA694053 | ENA

The nuclear matrix associating protein HNRNPU functions as a key regulator of 3D genome architecture [Hi-C]

2017-12-03 | GSE95112 | GEO