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Gene expression in pachytene spermatocytes and round spermatids depleted of HDAC3

ABSTRACT: Gene expression in pachytene spermatocytes and round spermatids depleted of HDAC3

PROVIDER: PRJNA641378 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12075560_1.fastq.gz	Fastqsanger.gz
	SRR12075560_2.fastq.gz	Fastqsanger.gz
	SRR12075561_1.fastq.gz	Fastqsanger.gz
	SRR12075561_2.fastq.gz	Fastqsanger.gz
	SRR12075562_1.fastq.gz	Fastqsanger.gz

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Project description:Expression profiling of isolated populations of prepachytene spermatocytes (LP), pachytene spermatocytes (RP) and spermatids (ST) from PWD and B6 was performed to study the genome wide variation in gene expression between two mouse subspecies. To evaluate the transcriptional difference between B6 and PWD in during meiosis, we compared their transcriptomes in sorted populations of pre-pachytene primary spermatocytes (Leptonema, Zygotene and Pachytene), pachytene spermatocytes (Mid-late pachytene and diplotene) and spermatids. Populations of pre-pachytene spermatocytes, pachytene spermatocytes and spermatids were isolated from PWD and B6 mice. All populations were isolated in duplicates from two animals. Hence, RNA was prepared from 12 samples (= 2 strains x 3 tissues x 2 replicas) and was then subject to labeling and hybridization on microarray chips.

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Changes of H3K9ac and H3K27ac recruitment and gene expression in pachytene spermatocytes and round spermatids depleted of HDAC3

Project description:Changes of H3K9ac and H3K27ac recruitment and gene expression in pachytene spermatocytes and round spermatids depleted of HDAC3

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