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Amplification of Germ Cells through Parthenogenesis Faithfully Maintain Genomic Imprinting [RNA-seq]

ABSTRACT: Amplification of Germ Cells through Parthenogenesis Faithfully Maintain Genomic Imprinting [RNA-seq]

PROVIDER: PRJNA645195 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12187529_1.fastq.gz	Fastqsanger.gz
	SRR12187529_2.fastq.gz	Fastqsanger.gz
	SRR12187530_1.fastq.gz	Fastqsanger.gz
	SRR12187530_2.fastq.gz	Fastqsanger.gz

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Amplification of Germ Cells through Parthenogenesis Faithfully Maintain Genomic Imprinting

Project description:Amplification of Germ Cells through Parthenogenesis Faithfully Maintain Genomic Imprinting

| PRJNA645194 | ENA

Amplification of Germ Cells through Parthenogenesis Faithfully Maintain Genomic Imprinting [RRBS]

Project description:Amplification of Germ Cells through Parthenogenesis Faithfully Maintain Genomic Imprinting [RRBS]

| PRJNA644638 | ENA

Amplification of Germ Cells through Parthenogenesis Faithfully Maintain Genomic Imprinting [RRBS]

Project description:We asked whether oocyte number could be amplified through parthenogenesis of mouse oocytes, without requiring creation of a paternal genome and a genetically unique genome. Parthenotes develop to a blastocyst-like stage, and from this parthenogenetic ESCs (pESCs) can be derived with high efficiency. Like ESCs, pESCs maintain unlimited self-renewal and pluripotency, as well as germline competence. Further, we demonstrate that their expression pattern of imprinted maternal genes resembles that observed in oocytes. pESCs can be directed to differentiate into primordial germ cell-like cells (PGCLCs) and form oocytes that produce fertile pups and reconstitute ovarian endocrine functions. The transcriptome and imprinting pattern of PGCLCs differentiated from pESCs more closely approximate those of in vivo produced embryonic PGCs, than PGCLCs produced from ESCs. Parthenogenesis offers a promising route for deriving PGCLCs and amplifying oocytes by faithfully maintaining maternal genes, without fertilization.

2021-12-22 | GSE153979 | GEO

Amplification of Germ Cells through Parthenogenesis Faithfully Maintain Genomic Imprinting [RNA-seq]

Project description:We perform E9.5 PGC RNA-seq for indentify the germ cell marker genes and imprinting genes expression.

2021-12-22 | GSE154144 | GEO

Amplification of Oocytes through Parthenogenesis

2019-11-28 | GSE141103 | GEO

Amplification of Oocytes through Parthenogenesis [RRBS]

2023-10-26 | GSE141172 | GEO

Amplification of Germ Cells through Parthenogenesis Faithfully Maintain Genomic Imprinting

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-12-22 | GSE154145 | GEO

Amplification of Oocytes through Parthenogenesis

Project description:Amplification of Oocytes through Parthenogenesis

| PRJNA592123 | ENA

Allelic histone-to-DNA methylation switch establishes secondary DMR to maintain noncanonical imprinting

Project description:Faithful maintenance of genomic imprinting is essential for mammalian development. While germline DNA methylation-dependent (canonical) imprinting is relatively stable during development, the recently discovered oocyte-derived H3K27me3-mediated noncanonical imprinting is mostly transient in early embryos with only a few genes maintain imprinted expression in the extraembryonic lineage. How these few noncanonical imprinted genes maintain their extraembryonic-specific imprinting is unknown. Here we report that maintenance of extraembryonic-specific noncanonical imprinting requires maternal allele-specific de novo DNA methylation (secondary differentially methylation regions; DMRs) at implantation. The secondary DMRs are located at the gene promoters with paternal allele-specific H3K4me3 preformed during preimplantation development. Importantly, genetic ablation of Eed and DNA methyltransferases revealed that both maternal H3K27me3 and zygotic Dnmt3a/3b are required for establishing secondary DMRs and for maintaining noncanonical imprinting. Thus, our study not only reveals the mechanism underlying maintenance of noncanonical imprinting, but also sheds light on how histone modifications in oocytes and preimplantation embryos may shape the secondary DMRs in post-implantation embryos.

2019-12-26 | GSE130115 | GEO

buffalo parthenogenesis TMT LC-MS/MS

Project description:Oocytes act as the genetic vector for zygote reprogramming, and stores a large amount of material that is required to regulate embryogenesis. However, to date, there is no report on the dynamic changes of maternal proteins and genes that occur during the early stages of the embryo, particularly studies that use proteomic techniques in buffalos. Here, an integrated single-cell RNA sequencing transcriptomic and quantitative proteomic analysis were employed to systematically explore the dynamic function of maternally-expressed proteins in parthenogenesis model of buffalo.The proteome of the buffalo was quantitatively analyzed during parthenogenesis of mature oocytes and the two-cell stage embryo. Of 1,908 quantified proteins, 123 differed significantly. The transcriptome was analyzed 8 stages (GV, MII, 2-cell,4-cell,8-cell,16-cell,morula,blastocyst)of buffalo using the single-cell RNA sequencing, and a total of 3567 unique genes were identified to be differently expressed between all consecutive stages of pre-implantation development. Bioinformatics studies and validated results indicated that maternal expression of the proteins possibly plays a role in the formation of cellular junctions firstly after parthenogenetic activation and the “maternal-to-zygotic transition” (MZT) process exists during parthenogenesis and occur between the 8-cell to 16-cell stage.

2018-07-17 | PXD008549 | Pride

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