Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression analysis of U937 cells during the differentiation by phorbol 12-myristate 13-acetate (PMA)

ABSTRACT: Gene expression analysis of U937 cells during the differentiation by phorbol 12-myristate 13-acetate (PMA)

PROVIDER: PRJNA649795 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR12355568.fastq.gz	Fastqsanger.gz
	SRR12355569.fastq.gz	Fastqsanger.gz
	SRR12355570.fastq.gz	Fastqsanger.gz
	SRR12355571.fastq.gz	Fastqsanger.gz

Items per page:

1 - 4 of 4

Similar Datasets

Gene expression analysis of U937 cells during the differentiation by phorbol 12-myristate 13-acetate (PMA)

Project description:U937 cells, human lyphoma cell line, differentiate to the macrophage-like cells by the phorbol 12-myristate 13-acetate (PMA). We investigated the regulation mechanisms of gene expression during the U937 differentiation by RNA-seq.

2020-11-30 | GSE155459 | GEO

Gene expression analysis of U937 cells during the differentiation by phorbol 12-myristate 13-acetate (PMA)

Project description:Gene expression analysis of U937 cells during the differentiation by phorbol 12-myristate 13-acetate (PMA)

| PRJNA649795 | ENA

Expression profiling of exposure to phorbol myristate acetate - PMA and FOXK2 depletion with siRNA

Project description:Expression profiling was done of exposure to phorbol myristate acetate -PMA and FOXK2 depletion by siRNA transfection. This was done on the human osteosarcoma U2OS cell line which stably expresses FOXK2. PMA is an inducer of AP1 activity.

2011-11-03 | E-MEXP-3107 | biostudies-arrayexpress

Effects of PMA on exon usage of HL60, THP-1, and U937 cells

Project description:Myeloid leukemia cell lines HL60, THP-1, and U937 undergo macrophage-like differentiation after treatment with phorbol ester. To explore genes whose exon usage was altered during macrophage differentiation, we compared exome of PMA-treated (differentated) and vehicle-treated (undifferentiated) myeloid cell lines. HL60, THP-1, and U937 cells were treated with either phorbol 12-myristate 13-acetate (PMA,30nM) or its vehicle (DMSO) for 3 days, and subjected to exome analysis using Affymetrix human exon 1.0ST arrays.

2013-09-25 | E-GEOD-20567 | biostudies-arrayexpress

Effects of gene scilencing of USP2 on expression profiles of HL60 cells (PMA 1day and LPS 4hrs)

Project description:So far, we have found phorbol 12-myristate 13-acetate (PMA) induced ubiquitin specific peptidase (USP) 2b isoform in myeloid leukemia cell lines such as HL60, THP-1, and U937. HL60, THP-1, and U937 undergoes differentiation into macrophage-like cells after stimulation with phorbol ester. To explore molecular function of USP2 in macrophages especially during lipopolysaccharide(LPS) response, we assess expression profiles of HL60-derivatives continuously expressing shRNA for USP2 and control shRNA.

2018-02-25 | GSE21004 | GEO

Effects of gene scilencing of USP2 on expression profiles of HL60 cells (PMA 3days and LPS 4hrs)

2018-02-25 | GSE20733 | GEO

Differential gene expression in human THP-1 cell line undifferentiated, 3 days or 8 days differentiated with phorbol myristate acetate (PMA)

Project description:We analysed the capacity of THP-1 cells (differentiated to macrophagoid cells) to recognize RNA sequences via pattern recognition receptors in vitro. Gene expression was analysed by RNA-Microarray. Cytokine production was analysed by ELISA assays. We used microarrays to investigate differential gene expression in THP-1 cell line undifferentiated in comparison with 3 days or 8 days differentiated with phorbol myristate acetate (PMA). Microarray analysis revealed differential gene expression patterns of THP-1 when differentiated.

2015-11-09 | GSE67264 | GEO

Differential gene expression in human THP-1 cell line undifferentiated, 3 days or 8 days differentiated with phorbol myristate acetate (PMA)

2015-11-09 | E-GEOD-67264 | biostudies-arrayexpress

Dose-dependent phorbol 12-myristate-13-acetate mediated monocyte-to-macrophage differentiation induces unique proteomic signatures in THP1 cells

Project description:Macrophages are sentinels of the immune system and THP1 monocytic cells are one of the widely used models to study immune responses in macrophages. Several monocyte-to-macrophage differentiation protocols exist with phorbol 12-myristate-13-acetate (PMA) being widely used and accepted. However, the concentrations and durations of PMA treatment to induce differentiation varies widely in published literature. In this study, we determined the proteome expression dynamics of THP1 macrophages differentiated from monocytes by three commonly used PMA-based differentiation protocols using dimethyl labeling-based quantitative proteomics analysis. Our analysis shows that variations in PMA concentration and varying periods of rest post-stimulation result in downstream differences in proteome expression and cellular processes. We demonstrate that these differences result in altered expression of cytokines upon stimulation with different TLR ligands. Together, these findings provide a valuable resource that significantly expands the knowledge of protein expression dynamics in in vitro models of macrophages which in turn has profound impact on the immune responses being studied.

2021-09-08 | PXD015872 | Pride

RNA-seq analysis of PMA treament with or without LPS on human monocytic cell line THP1

Project description:Transcriptome analysis of the human monocytic cell line THP1 (ATCC TIB-202) treated or not with phorbol myristate acetate (PMA) +/- lipopolysaccharides-lipopolysaccharides binding protein (LPS-LBP)

2018-07-17 | GSE117162 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data