Project description:An integrated approach was used to identify genes associated with resistance to Aeromonas hydrophila, an important bacterial pathogen causing aeromoniasis in rohu Labeo rohita. DNA polymorphism and gene expression profiling in lines of L. rohita selected for resistance or susceptibility to challenge with A. hydrophila, and grown in a common environment, were studied using Illumina mRNA-seq of selectively pooled RNA samples.
Project description:An integrated approach was used to identify genes associated with resistance to Aeromonas hydrophila, an important bacterial pathogen causing aeromoniasis in rohu Labeo rohita. DNA polymorphism and gene expression profiling in lines of L. rohita selected for resistance or susceptibility to challenge with A. hydrophila, and grown in a common environment, were studied using Illumina mRNA-seq of selectively pooled RNA samples. mRNA-seq of pooled samples from resistant and susceptible lines of rohu for expression characterisation and SNP detection
Project description:The aim of this study was to develop a Peptide Atlas repository for Labeo rohita. Using LC-MS/MS, we have performed in-depth proteomics analysis of 19 different sample types, including 17 tissue samples, plasma from female fish and embryo 4-day post fertilization. Whole analysis resulted in the identification of more than 6015 confident canonical proteins along with ~3000 non canonical peoteins corresponding to more than 150 thousands peptides. This proteomic dataset would complement the recently published genome to accelerate further research.
