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Single-cell sequencing of glioblastoma reveals central nervous system susceptibility to SARS-CoV-2

ABSTRACT: Single-cell sequencing of glioblastoma reveals central nervous system susceptibility to SARS-CoV-2

PROVIDER: PRJNA661274 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12581863_1.fastq.gz	Fastqsanger.gz
	SRR12581863_2.fastq.gz	Fastqsanger.gz
	SRR12581864_1.fastq.gz	Fastqsanger.gz
	SRR12581864_2.fastq.gz	Fastqsanger.gz
	SRR12581865_1.fastq.gz	Fastqsanger.gz

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Single-cell sequencing of glioblastoma reveals central nervous system susceptibility to SARS-CoV-2

Project description:Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) caused the recent global COVID-19 outbreak, which led to a public health emergency. Entry of SARS-CoV-2 into human cells is dependent on the SARS-CoV receptor, angiotensin converting enzyme 2 (ACE2) receptor, and cathepsin. Cathepsin degrades the spike protein (S protein), which results in the entry of viral nucleic acid into the human host cell. Methods: We explored the susceptibility of the central nervous system (CNS) to SARS-CoV-2 infection using single-cell transcriptome analysis of glioblastoma. Results: The results showed that ACE2 expression is relatively high in endothelial cells (ECs), bone marrow mesenchymal stem cells (BMSCs), and neural precursor cells (NPCs). Cathepsin B (Cat B) and cathepsin (Cat L) were also strongly expressed in various cell clusters within the glioblastoma microenvironment. Immunofluorescence staining of glioma and normal brain tissue chips further confirmed that ACE2 expression co-localized with CD31, CD73, and nestin, which confirmed the susceptibility to SARS-CoV-2 of nervous system cells, including ECs, BMSCs and NPCs, from clinical specimens. Conclusions: These findings reveal the mechanism of SARS-CoV-2 neural invasion and suggest that special attention should be paid to SARS-CoV-2-infected patients with neural symptoms, especially those who suffered a glioma.

2020-11-12 | GSE157424 | GEO

Single-Cell Sequencing of Glioblastoma Reveals Central Nervous System Susceptibility to SARS-CoV-2.

Project description:BackgroundSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) caused the recent global COVID-19 outbreak, which led to a public health emergency. Entry of SARS-CoV-2 into human cells is dependent on the SARS-CoV receptor, angiotensin converting enzyme 2 (ACE2) receptor, and cathepsin. Cathepsin degrades the spike protein (S protein), which results in the entry of viral nucleic acid into the human host cell.MethodsWe explored the susceptibility of the central nervous system (CNS) to SARS-CoV-2 infection using single-cell transcriptome analysis of glioblastoma.ResultsThe results showed that ACE2 expression is relatively high in endothelial cells (ECs), bone marrow mesenchymal stem cells (BMSCs), and neural precursor cells (NPCs). Cathepsin B (Cat B) and cathepsin (Cat L) were also strongly expressed in various cell clusters within the glioblastoma microenvironment. Immunofluorescence staining of glioma and normal brain tissue chips further confirmed that ACE2 expression co-localized with CD31, CD73, and nestin, which confirmed the susceptibility to SARS-CoV-2 of nervous system cells, including ECs, BMSCs, and NPCs, from clinical specimens.ConclusionsThese findings reveal the mechanism of SARS-CoV-2 neural invasion and suggest that special attention should be paid to SARS-CoV-2-infected patients with neural symptoms, especially those who suffered a glioma.

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2010-11-13 | E-GEOD-25299 | biostudies-arrayexpress

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SARS-CoV-2 Invades the Central Nervous System via the Olfactory Route in Rhesus Monkeys

Project description:SARS-CoV-2 Invades the Central Nervous System via the Olfactory Route in Rhesus Monkeys

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