Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

YAP contributes to DNA methylation remodeling upon mouse embryonic stem cell differentiation

ABSTRACT: YAP contributes to DNA methylation remodeling upon mouse embryonic stem cell differentiation

PROVIDER: PRJNA662507 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

YAP contributes to DNA methylation remodeling upon mouse embryonic stem cell differentiation.

Project description:The Yes-associated protein (YAP), one of the major effectors of the Hippo pathway together with its related protein WW-domain-containing transcription regulator 1 (WWTR1; also known as TAZ), mediates a range of cellular processes from proliferation and death to morphogenesis. YAP and WW-domain-containing transcription regulator 1 (WWTR1; also known as TAZ) regulate a large number of target genes, acting as coactivators of DNA-binding transcription factors or as negative regulators of transcription by interacting with the nucleosome remodeling and histone deacetylase complexes. YAP is expressed in self-renewing embryonic stem cells (ESCs), although it is still debated whether it plays any crucial roles in the control of either stemness or differentiation. Here we show that the transient downregulation of YAP in mouse ESCs perturbs cellular homeostasis, leading to the inability to differentiate properly. Bisulfite genomic sequencing revealed that this transient knockdown caused a genome-wide alteration of the DNA methylation remodeling that takes place during the early steps of differentiation, suggesting that the phenotype we observed might be due to the dysregulation of some of the mechanisms involved in regulation of ESC exit from pluripotency. By gene expression analysis, we identified two molecules that could have a role in the altered genome-wide methylation profile: the long noncoding RNA ephemeron, whose rapid upregulation is crucial for the transition of ESCs into epiblast, and the methyltransferase-like protein Dnmt3l, which, during the embryo development, cooperates with Dnmt3a and Dnmt3b to contribute to the de novo DNA methylation that governs early steps of ESC differentiation. These data suggest a new role for YAP in the governance of the epigenetic dynamics of exit from pluripotency.

| S-EPMC7948423 | biostudies-literature

YAP contributes to DNA methylation remodeling upon mouse embryonic stem cell differentiation

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-12-08 | GSE157707 | GEO

Analysis of hippo pathway upon YAP knockdown and MEK inhibition

Project description:This experiment analyzes the global proteome and phosphoproteome in YAP amplified cell lines upon YAP knockdown and/or MEK inhibition.

2020-03-17 | MSV000085111 | MassIVE

RNA-Seq of DU145 cell line upon knockdown of YAP, TAZ, or YAP/TAZ

Project description:Transcriptome analysis of prostate cancer patient derived organoid DU145 cell line upon knockdown of YAP, TAZ, or YAP/TAZ mediated by siRNAs

2022-03-24 | GSE199347 | GEO

RNA-Seq of MSK-PCa3 prostate cancer organoids upon knockdown of FOSL1, YAP, TAZ, or YAP/TAZ

Project description:Transcriptome analysis of prostate cancer patient derived organoid MKS-PCa3 upon knockdown of FOSL1, YAP, TAZ, or YAP/TAZ mediated by siRNAs

2022-02-20 | GSE196813 | GEO

Remodeling of Zn2+ Homeostasis Upon Differentiation of Mammary Epithelial Cells

Project description:Remodeling of Zn2+ Homeostasis Upon Differentiation of Mammary Epithelial Cells

| PRJNA602910 | ENA

Expression profile of mouse embryonic stem cells upon knockdown or overexpression of YAP

Project description:We looked at the expression profiles of embyonic stem cells (ESCs) 48 hours after YAP siRNAs transfection, to knockdown YAP expression, or after pCAG-YAP transfection, to over-express YAP protein. Total RNA was isolated from three independent experiments and analyzed by RNA-seq.

2020-12-08 | GSE157706 | GEO

Trascriptional profile of BRAF inhibitor-resistant SKMEL28 and WM3248 melanoma cells and changes upon YAP/TAZ siRNA knockdown

Project description:The activation of transcriptional coactivators YAP and its paralog TAZ has been shown to promote resistance to anti-cancer therapies. YAP/TAZ activity is tightly coupled to actin cytoskeleton architecture. However, the influence of actin remodeling on cancer drug resistance remains largely unexplored. Here, we report a pivotal role of actin remodeling in YAP/TAZ-dependent BRAF inhibitor resistance in BRAF V600E mutant melanoma cells. Melanoma cells resistant to BRAF inhibitor PLX4032 exhibit an increase in actin stress fiber formation, which appears to promote the nuclear accumulation of YAP/TAZ. Knockdown of YAP/TAZ overcomes PLX4032 resistance, whereas overexpression of constitutively active YAP induces resistance. Moreover, inhibition of actin polymerization and cytoskeletal tension in melanoma cells suppresses both YAP/TAZ activation and PLX4032 resistance. Our siRNA library screening identifies actin dynamics regulator TESK1 as a novel vulnerable point of the YAP/TAZ-dependent resistance pathway. These results suggest that inhibition of actin remodeling is a promising synthetic lethal strategy to suppress resistance in BRAF inhibitor therapies.

2015-11-18 | GSE68599 | GEO

Methylation pattern profile of mouse embryonic stem cells upon knockdown of YAP, during neuroectodermal differentiation

Project description:We analyzed by BS-seq the methylation pattern of CTR and YAP knockdown cells in undifferentiated (T0) embryonic stem cells (ESCs) vs cells differentiated toward neuroectodermal fate at day 4 of differentiation (T4). Two biological duplicates for each condition.

2020-12-08 | GSE157704 | GEO

Methylation pattern profile of mouse embryonic stem cells upon knockdown of YAP, during neuroectodermal differentiation

Project description:Methylation pattern profile of mouse embryonic stem cells upon knockdown of YAP, during neuroectodermal differentiation

| PRJNA662504 | ENA