Project description:To investigate the relationship between the resistance of male and female Penaeus vannamei and their immunity， we collected hemocytes from shrimps stimulated by Vibrio parahaemolyticus.
Project description:We provided a step forward in examining the transcriptomic response of L. vannamei to different salinity conditions following the application of the next-generation sequencing techniques and was first to explain osmoregulation of L. vannamei at a transcriptome-wide scale based on three practical cultivated models with different salinities. The assembled sequence data comprising 349,012 unique transcripts were obtained, and the established transcriptional database provided a comprehensive sequence source for this non-model organism.
2019-11-20 | GSE64596 | GEO
Project description:the biofloc-based shrimp culture systems
Project description:Escherichia coli culture was subjected to two different types of nutritional scenarios, abundant carbon/ nitrogen sources and scarce carbon/nitrogen medium. Study revealed that scarce medium adapted culture were more tolerant to hydrogen peroxide than abundant medium.
Project description:Aspergillus niger produces a variety of lignocellulolytic enzymes (cellulases, hemicellulases, among others) and is regarded as cell factory for the production of heterologous proteins. Therefore, there is a growing interest in the study of its genes and the understanding of the cellular mechanisms in order to expand its applications. On the other hand, we have shown that enzyme production by A. niger is higher when grown forming biofilms than when grown conventionally in submerged systems. The objective of this study was to perform a global transcriptomic analysis and an expression analysis of both lignocellulases and biofilm regulatory genes as compared to A. niger in submerged culture. DNA microarray assays were performed to investigate the global gene expression which yielded information on the expression of more than 90% of A. niger genes. To further this comparison, the two culture systems were supplemented with different carbon sources (glucose, lactose, xylose and maltose) to establish a differential gene expression under different culture conditions. Also, to validate the differential expression qPCR was performed for quantitative comparison of the transcriptional level of genes in both culture systems.
Project description:The unicellular microalga Dunaliella salina is one of the halotolerant and cell wall-less green microalgae in Dunaliella genus. The ability of halotolerance in Dunaliella is attributed to the accumulation of glycerol. Both sugar made by photosynthesis and starch serve as carbon sources for glycerol biosynthesis. Quantitative PCR-based analyses concluded no apparent transcriptional regulation of glycerol, carbon fixation, and starch metabolisms upon salinity stresses. To examine whether or not transcriptional regulation is involved at the transcriptomic level, we assembled a de novo deep sequencing transcriptome. By using a pathway-based approach, we show that low- and high-salt (i.e., 0.5M versus 2M NaCl) adapted cells share a common transcriptomic profile and that subsets of ESTs associated with energy metabolisms are less affected upon salinity stress. We find that enzymes involved in glycerol, carbon fixation, and starch metabolisms are encoded by multiple EST isoforms. We show that EST isoforms encoding dihydroacetone reductase in glycerol metabolism, phosphoglycerate kinase in carbon fixation, and beta-amylase and fructobiphosphate aldolase in starch metabolism display a correlated transcriptional level change to the alteration of glycerol and starch contents upon salinity stresses. Taken together, our results demonstrate that some enzymes involved in glycerol, carbon fixation, and starch metabolisms are regulated at the transcriptional level upon salinity stresses. Furthermore, our analyses indicate that energy metabolisms are not drastically affected upon salinity stresses, consistent with its ability to adapt to a wide range of salinities.
2017-02-22 | GSE74466 | GEO
Project description:Microbial degradation of different carbon sources
Project description:The goals of this study are to compare different gene expressions for Penicillium oxalicum wild type strain (WT), and Podot1 knockout strain (ΔPodot1) in different carbon sources. The deletion of Podot1 downregulated genes involved in the septin complex, extracellular region, and interspecies interaction between organisms when strains were cultivated with 2% glucose as carbon sources, and downregulated genes involved in cellulase activity, cellulose binding, glucosidase activity, and polysaccharide catabolic process when strains were cultivated with 1% microcrystalline cellulose and 1% wheat bran as carbon sources. We find the extracellular region was downregulated under both different carbon sources in ΔPodot1. This study provides the information that PoDot1 function are required in mycelial development and hydrolase activity of P. oxalicum.
