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Amino acids activate mTORC1 to release roe deer embryos from decelerated proliferation during diapause [Luminal epithelium]

ABSTRACT: Amino acids activate mTORC1 to release roe deer embryos from decelerated proliferation during diapause [Luminal epithelium]

PROVIDER: PRJNA666550 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12746100.fastq.gz	Fastqsanger.gz
	SRR12746101.fastq.gz	Fastqsanger.gz
	SRR12746102.fastq.gz	Fastqsanger.gz
	SRR12746103.fastq.gz	Fastqsanger.gz
	SRR12746104.fastq.gz	Fastqsanger.gz

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Amino acids activate mTORC1 to release roe deer embryos from decelerated proliferation during diapause [Luminal epithelium]

Project description:mRNA sequencing of diapausing and embryos that resumed a fast rate of development to identify molecular factors involved in the regulation of embryonic diapause

2021-08-15 | GSE158784 | GEO

Amino acids activate mTORC1 to release roe deer embryos from decelerated proliferation during diapause

Project description:Amino acids activate mTORC1 to release roe deer embryos from decelerated proliferation during diapause

| PRJNA666623 | ENA

Amino acids activate mTORC1 to release roe deer embryos from decelerated proliferation during diapause [Embryo]

Project description:mRNA sequencing of diapausing and embryos that resumed a fast rate of development to identify molecular factors involved in the regulation of embryonic diapause

2021-08-15 | GSE158805 | GEO

Amino acids activate mTORC1 to release roe deer embryos from decelerated proliferation during diapause [Embryo]

Project description:Amino acids activate mTORC1 to release roe deer embryos from decelerated proliferation during diapause [Embryo]

| PRJNA666626 | ENA

Amino acids activate mTORC1 to release roe deer embryos from decelerated proliferation during diapause.

Project description:Embryonic diapause in mammals leads to a reversible developmental arrest. While completely halted in many species, European roe deer (Capreolus capreolus) embryos display a continuous deceleration of proliferation. During a 4-mo period, the cell doubling time is 2 to 3 wk. During this period, the preimplantation blastocyst reaches a diameter of 4 mm, after which it resumes a fast developmental pace to subsequently implant. The mechanisms regulating this notable deceleration and reacceleration upon developmental resumption are unclear. We propose that amino acids of maternal origin drive the embryonic developmental pace. A pronounced change in the abundance of uterine fluid mTORC1-activating amino acids coincided with an increase in embryonic mTORC1 activity prior to the resumption of development. Concurrently, genes related to the glycolytic and phosphate pentose pathway, the TCA cycle, and one carbon metabolism were up-regulated. Furthermore, the uterine luminal epithelial transcriptome indicated increased estradiol-17β signaling, which likely regulates the endometrial secretions adapting to the embryonic needs. While mTORC1 was predicted to be inactive during diapause, the residual embryonic mTORC2 activity may indicate its involvement in maintaining the low yet continuous proliferation rate during diapause. Collectively, we emphasize the role of nutrient signaling in preimplantation embryo development. We propose selective mTORC1 inhibition via uterine catecholestrogens and let-7 as a mechanism regulating slow stem cell cycle progression.

| S-EPMC8536382 | biostudies-literature

Amino acids activate mTORC1 to release roe deer embryos from decelerated proliferation during diapause

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-08-15 | GSE158806 | GEO

Metabolic control over mTOR dependent diapause-like state

Project description:Regulation of embryonic diapause, dormancy that interrupts the tight connection between develop- mental stage and time, is still poorly understood. Here, we characterize the transcriptional and metabolite profiles of mouse diapause embryos and identify unique gene expression and metabolic signatures with activated lipolysis, glycolysis, and metabolic pathways regulated by AMPK. Lipolysis is increased due to mTORC2 repression, increasing fatty acids to support cell survival. We further show that starvation in pre-implantation ICM-derived mouse ESCs induces a reversible dormant state, transcriptionally mimicking the in vivo diapause stage. During starvation, Lkb1, an upstream kinase of AMPK, represses mTOR, which induces a revers- ible glycolytic and epigenetically H4K16Ac-negative, diapause-like state. Diapause furthermore activates expression of glutamine transporters SLC38A1/2. We show by genetic and small molecule inhibitors that glutamine transporters are essential for the H4K16Ac-negative, diapause state. These data sug- gest that mTORC1/2 inhibition, regulated by amino acid levels, is causal for diapause metabolism and epigenetic state.

2020-01-14 | GSE143494 | GEO

Next generation sequencing of Coccinella septempunctata L. with non-diapause, diapause, and diapause-terminated states

Project description:The most commonÂ ladybird beetle, Coccinella septempunctata L., is an excellent predator of crop pests such as aphids and white flies, and it shows a wide range of adaptability, a large appetite and a high reproductive ability. In this study, we collected female adults in three different states, i.e., non-diapause, diapause and diapause termination, for transcriptome sequencing. The experimental insects consisted of three different states as follows: Non-diapause female insects were reared at 24Â±1Â°C, with a RH of 70Â±10% and a 16:8 h light: dark (L: D) photoperiod and collected after their first oviposition. Female adults in diapause were reared at 18Â±1Â°C at an RH of 70Â±10% and a 10:14-h (L:D) photoperiod. The experimental diapause insects were collected after 30 days. Diapause-terminated adults were transferred to another climatic cabinet with the 30-day diapause insects and reared under the same conditions as the non-diapause insects. After their first oviposition, the female insects were collected and stored at -80Â°C. Three biological replicates per treatment (non-diapause, diapause, diapause-terminated) were sequenced using Illumina HiSeq 2500.

2016-06-01 | E-GEOD-75645 | biostudies-arrayexpress

Methylation studies in roe deer

Project description:An Infinium microarray platform (GPL28271, HorvathMammalMethylChip40) was used to generate DNA methylation data from blood samples of roe deer n=94 blood samples

2022-08-02 | GSE184216 | GEO

Transcriptional shut-off of MAP kinase signaling enables pluripotency maintenance during diapause [RNA-seq]

Project description:Exposure of unicellular or multicellular organisms to adverse environmental conditions, including nutrient deprivation, may induce a state of suspended animation or diapause. We here report that broad repression of RNA Pol II-driven gene expression by inhibiting the BET family's bromodomain-containing proteins (BET) triggers diapause in mouse embryonic stem (ES) cells. The diapause ES cells upregulate a functionally linked group of genes encoding negative regulators of MAP kinase signaling (NRMKS), which play a crucial role in ES cell differentiation. The elevated NRMKS expression is a hallmark of cells exposed to distinct diapause-inducing conditions, including mTOR inhibition, and are required for the pluripotency maintenance during diapause. Mechanistically, inhibition of mTORC1/2 leads to rapid decline of the Capicua transcriptional repressor (CIC) at the NRMKS gene promoters, followed by rapid transcriptional NRMKS gene upregulation. The mTOR and BET-dependent transcriptional switch supporting the undifferentiated state of the diapause ES cells suggests a broader usage of this mechanism in maintaining the undifferentiated state of metabolically dormant stem- or stem-like cells in different tissues.

2024-10-08 | GSE249389 | GEO

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