Project description:Alicyclobacillus acidoterrestris is a major putrefying bacterium that can cause pecuniary losses in the global juice industry. Current detection approaches are time-consuming and exhibit reduced specificity and sensitivity. In this study, an immunoproteomic approach was utilized to identify specific biomarkers from A. acidoterrestris for the development of new detection methods. Cell surface-associated proteins were extracted and separated by 2-D (two-dimensional) gel electrophoresis. Immunogenic proteins were detected by Western blot analysis using antisera against A. acidoterrestris. Twenty-two protein spots exhibiting immunogenicity were excised and eighteen of the associated spots were successfully identified by matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (MALDI-TOF/TOF MS). These proteins were observed to be involved in energy and carbohydrate metabolism, transmembrane transport, response to oxidative stress, polypeptide biosynthesis, and molecule binding activity. This is the first report detailing the identification of cell surface-associated antigens of A. acidoterrestris. The identified immunogenic proteins could serve as potential targets for the development of novel detection methods.

| S-EPMC6288362 | biostudies-literature

Gene expression during metabolism of sugars by Alicyclobacillus acidocaldarius

Project description:The purpose of this study was to characterize carbon metabolism and gene regulation in Alicyclobacillus acidocaldarius during growth on monosaccharides in an effort to determine whether carbon catabolite repression was active. Chemostat studies and global transcriptome analysis were used to accomplish this goal.

2016-08-31 | GSE84032 | GEO

Alicyclobacillus acidocaldarius strain:CU92

Project description:Alicyclobacillus acidocaldarius genome sequencing

| PRJNA280536 | ENA

Alicyclobacillus tengchongensis strain:CGMCC1504

Project description:Alicyclobacillus tengchongensis strain:CGMCC1504 Genome sequencing and assembly

| PRJNA271324 | ENA

Alicyclobacillus sp. SP_1 strain:SP_1

Project description:Alicyclobacillus sp. SP_1 strain:SP_1 Genome sequencing

| PRJNA836356 | ENA

Gene expression during metabolism of polysaccharides and sugars by Alicyclobacillus acidocaldarius

Project description:The purpose of this study was to characterize carbon metabolism and gene regulation in Alicyclobacillus acidocaldarius during growth on wheat arabinoxylan and the effect of pentose and hexose sugars on gene expression Chemostat studies and global transcriptome analysis were used to accomplish this goal.

2017-01-01 | GSE89078 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data