Project description:Transcriptional profiling of Arabidopsis rossette leaves comparing WT Col-0 with a transgenic line overexpressing AhDGR gene from Amaranthus hypochondriacus.

2016-11-30 | GSE84726 | GEO

Transgenic Ah24-OE vs Wild type Col 0 Arabidopsis plants

Project description:Transcriptional profiling of Arabidopsis rossette leaves comparing WT Col-0 with a transgenic line overexpressing Ah24 gene from Amaranthus hypochondriacus.

2015-06-26 | GSE70272 | GEO

Transgenic AhNF-YC-OE vs Wild type Col 0 Arabidopsis plants

2015-07-14 | GSE70861 | GEO

Transgenic AhNF-YC-OE vs Wild type Col 0 Arabidopsis plants

Project description:Transcriptional profiling of Arabidopsis rossette leaves comparing WT Col-0 with a transgenic lines overexpressing AhNF-YC gene from Amaranthus hypochondriacus in three different conditions. Three-condition experiment WT vs AhNF-YC OE plant leaves. The analyzed conditions were: normal growth conditions, water stress for 5 days and 24 hrs of recovery after normal watering was reestablished to 8-day water stressed plants.

2015-07-14 | E-GEOD-70861 | biostudies-arrayexpress

mRNA sequencing from Arabidopsis mutants and transgenic plants in the Col-0 accession

Project description:We sequenced the mRNA of Arabidopsis mutants and transgenic plants with modified RTL genes

2019-12-19 | GSE142272 | GEO

Transgenic Ah24-OE vs Wild type Col 0 Arabidopsis plants

Project description:Transcriptional profiling of Arabidopsis rossette leaves comparing WT Col-0 with a transgenic line overexpressing Ah24 gene from Amaranthus hypochondriacus. One-condition experiment WT vs Ah24 OE plant leaves.

2015-06-26 | E-GEOD-70272 | biostudies-arrayexpress

Arabidopsis thaliana: transgenic plants vs wild type

Project description:Transcriptional profiling of Arabidopsis thaliana 12-days old seedlings comparing Col-0 wild type with transgenic plants with altered expression of dual-targetting plastid/mitochondrial organellar RNA-polymerase RPOTmp. Transgenic plants used for experiment were: overexpressor plants obtained by transformation of Col-0 WT plants with genetic constructs created in [Tarasenko et al., 2016] contained catalytic part of RPOTmp enzyme with transit peptides of RPOTm (mitochondrial) and RPOTp (plastid) by agrobacterial transformation; plants with complementation of RPOTmp functions in mitochondria or chloroplasts obtained from transformation of GABI_286E07 rpotmp knockout-mutant plants with genetic constructs created in [Tarasenko et al., 2016]. Goal was to determine the effects of RPOTmp knockout/overexpression on global Arabidopsis thaliana gene expression.

2023-12-25 | GSE251661 | GEO

Expression profiles of MicroRNA and SiRNA of Arabidopsis thaliana Col-0 and transgenic plants

Project description:Expression profiles of MicroRNA and SiRNA of Arabidopsis thaliana Col-0 and transgenic plants with constitutive expression of the chimeric receptors NRG1 grown at different temperature To reveal the underlying molecular mechanism of de-cosuppression with memory by high temperature in Arabidopsis, we performed the expression profiles of microRNA and SiRNA in transgenic plants with constitutive expression of the chimeric receptors NRG1 and wide type Col-0 grown at different temperature using the Custom LC Sciences Arabidopsis microRNA and SiRNA array. Keywords: high temperature, de-cosuppression, MicroRNA, SiRNA Total RNA was prepared from wild type and NRG1 plants grown at 22ºC and 30ºC for 4 weeks with TRIzol reagent according to the manufacturer’s protocol (Invitrogen). Small RNAs (<300nt) were isolated using a YM-100 Microcon centrifugal filter (Millipore), and 3’-extended with a poly(A) tail for later fluorescent dye staining. Two different tags were used for the two RNA samples labeled with Cy3 or Cy5 fluorescent dyes in dual-sample experiments. Also dye-swap experiments were carried out to control for labeling bias. Hybridization was performed overnight on a µParafloTM microfluidic chip. Each miRNA and siRNA had 6 and 3 repeat prints, respectively. Six replicate arrays were performed with six independent pairs of RNA samples. After hybridization, array images were obtained using an Axon GenePix 4000B laser scanner and digitized using the Array-Pro image software (Media Cybernetics). The data were corrected by subtracting the background and normalized using the LOWESS (locally weighted regression) method. All data calculations were performed by LC Science (Houston, TX).

2008-05-24 | E-GEOD-11535 | biostudies-arrayexpress

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