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Symmetry breaking of tissue mechanics in wound induced hair follicle regeneration [bulk RNA-seq]

ABSTRACT: Symmetry breaking of tissue mechanics in wound induced hair follicle regeneration [bulk RNA-seq]

PROVIDER: PRJNA670922 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12884030_1.fastq.gz	Fastqsanger.gz
	SRR12884030_2.fastq.gz	Fastqsanger.gz
	SRR12884031_1.fastq.gz	Fastqsanger.gz
	SRR12884031_2.fastq.gz	Fastqsanger.gz
	SRR12884032_1.fastq.gz	Fastqsanger.gz

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Symmetry breaking of tissue mechanics in wound induced hair follicle regeneration

Project description:Symmetry breaking of tissue mechanics in wound induced hair follicle regeneration

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Symmetry breaking of tissue mechanics in wound induced hair follicle regeneration

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Symmetry breaking of tissue mechanics in wound induced hair follicle regeneration [scRNA-seq]

Project description:Tissue regeneration is a process that recapitulates the molecular and mechanical aspects of development and evolution. We use the wound-induced hair neogenesis (WIHN) model to investigate the mechanical and molecular responses of the laboratory (Mus) and African spiny (Acomys) mice. Laboratory and spiny mice showed an opposite trend of spatiotemporal morphogenetic field for WIHN during wound healing, and wound stiffness gradient across the whole wound bed predicated pattern of hair formation. Using bulk and single-cell RNA-seq analysis and K14-Cre-Twist1 transgenic mice, we identified the central role of the Twist1 pathway as the mediator of epidermal-dermal interaction and the emergence of periodic hair primordia. Lastly, we generated a Turing model with an underlying measure of stiffness to support a two-scale tissue mechanic model to explain the setup of a morphogenetic field from a wound bed (mm scale) or periodically arranged hair primordia from a morphogenetic field (μm scale). Delineating the common and distinct chemo-mechanical events during regenerative wound healing between laboratory and African spiny mice reveal its evo-devo advantages, which provide new perspectives for regenerative medicine.

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Symmetry breaking of tissue mechanics in wound induced hair follicle regeneration [bulk RNA-seq]

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Symmetry breaking of tissue mechanics in wound induced hair follicle regeneration of laboratory and spiny mice.

Project description:Tissue regeneration is a process that recapitulates and restores organ structure and function. Although previous studies have demonstrated wound-induced hair neogenesis (WIHN) in laboratory mice (Mus), the regeneration is limited to the center of the wound unlike those observed in African spiny (Acomys) mice. Tissue mechanics have been implicated as an integral part of tissue morphogenesis. Here, we use the WIHN model to investigate the mechanical and molecular responses of laboratory and African spiny mice, and report these models demonstrate opposing trends in spatiotemporal morphogenetic field formation with association to wound stiffness landscapes. Transcriptome analysis and K14-Cre-Twist1 transgenic mice show the Twist1 pathway acts as a mediator for both epidermal-dermal interactions and a competence factor for periodic patterning, differing from those used in development. We propose a Turing model based on tissue stiffness that supports a two-scale tissue mechanics process: (1) establishing a morphogenetic field within the wound bed (mm scale) and (2) symmetry breaking of the epidermis and forming periodically arranged hair primordia within the morphogenetic field (μm scale). Thus, we delineate distinct chemo-mechanical events in building a Turing morphogenesis-competent field during WIHN of laboratory and African spiny mice and identify its evo-devo advantages with perspectives for regenerative medicine.

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Self-organization and symmetry breaking in intestinal organoid development [scRNA-Seq]

Project description:Intestinal organoids are complex three-dimensional structures that mimic cell type composition and tissue organization of the intestine by recapitulating the self-organizing capacity of cell populations derived from a single stem cell. Crucial in this process is a first symmetry-breaking event, in which only a fraction of identical cells in a symmetrical cyst differentiate into Paneth cells, which in turn generates the stem cell niche and leads to asymmetric structures such as crypts and villi. We here combine a quantitative single-cell gene expression and imaging approach to characterize the development of intestinal organoids from a single cell. We show that intestinal organoid development follows a regeneration process driven by transient Yap1 activation. Cell-to-cell variability in Yap1, emerging in symmetrical cysts, initiates a Notch/Dll1 lateral inhibition event driving the symmetry-breaking event and the formation of the first Paneth cell. Our findings reveal how single cells exposed to a uniform growth-promoting environment have the intrinsic ability to generate emergent, self-organized behavior resulting in the formation of complex multicellular asymmetric structures.

2019-04-25 | GSE115956 | GEO

Self-organization and symmetry breaking in intestinal organoid development [bulk RNA-Seq]

2019-04-25 | GSE115955 | GEO

Self-organization and symmetry breaking in intestinal organoid development

Project description:Self-organization and symmetry breaking in intestinal organoid development

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Controlling organoid symmetry breaking uncovers an excitable system underlying human axial elongation

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