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Identify the role of host ATF4 on CAFs in the tumor microenvironment of large size and small size B16F10 tumors

ABSTRACT: Identify the role of host ATF4 on CAFs in the tumor microenvironment of large size and small size B16F10 tumors

PROVIDER: PRJNA671500 | ENA |

REPOSITORIES: ENA

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Identify the role of host ATF4 on CAFs in the tumor microenvironment of large size B16F10 tumors

Project description:To delineate the role of host ATF4 in the sequence of events leading to tumor growth, we performed transcriptional profiling at the single-cell level (scRNA-seq) in larger (300 mm3) size B16F10 tumors grown in Atf4wt/wt and Atf4D/D mice. We surprisingly detected impaired CAFs activation in Atf4D/D mice, based on the expression levels of Acta2 and Pdgfrb, as one of the most commonly used CAF markers. Among our key findings, we identified the vascular CAFs (vCAFs), a spatially distinct CAF subcluster featured by the highest levels of aSMA and PDGFRb, that were reduced in Atf4D/D mice.

2022-05-10 | GSE159994 | GEO

Identify the role of host ATF4 on CAFs in the tumor microenvironment of small size B16F10 tumors

Project description:To delineate the role of host ATF4 in the sequence of events leading to tumor growth, we performed transcriptional profiling at the single-cell level (scRNA-seq) in smaller (150 mm3) size B16F10 tumors grown in Atf4wt/wt and Atf4D/D mice. Among others, we observed striking differences in gene expression in a cluster that corresponds to CAFs. In this cluster, we identified 148 DE genes, including a significant downregulation of Col1a1 and Col1a2 in the Atf4D/D mice-grown tumors. Notably, the expression levels of aSMA and Pdgfrb were nearly absent in the tumors grown in Atf4D/D mice. Finally, after CAFs subclustering, we identified the vCAFs (vascular CAFs) that are considered essential for vascular development and angiogenesis to be significantly reduced in the Atf4D/D mice.

2022-05-10 | GSE159995 | GEO

Identify the role of host ATF4 on CAFs in the tumor microenvironment of large size B16F10 tumors

Project description:Identify the role of host ATF4 on CAFs in the tumor microenvironment of large size B16F10 tumors

| PRJNA671506 | ENA

Identify the role of host ATF4 on CAFs in the tumor microenvironment of small size B16F10 tumors

Project description:Identify the role of host ATF4 on CAFs in the tumor microenvironment of small size B16F10 tumors

| PRJNA671507 | ENA

Identify the role of host ATF4 on CAFs in the tumor microenvironment of large size and small size B16F10 tumors

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-05-10 | GSE159996 | GEO

Expression data from gemcitabine treated pancreatic CAFs

Project description:Pancreatic ductal adenocarcinoma (PDAC) has a characteristically dense stroma comprised predominantly of cancer associated fibroblasts (CAFs). CAFs promote tumor growth, metastasis and treatment resistance. We aimed to investigate the molecular changes and functional consequences associated with chemotherapy treatment of PDAC CAFs. Chemoresistant immortalized CAFs (R-CAFs) were generated by continuous incubation in 100nM gemcitabine. Gene expression differences between treatment naÃ¯ve CAFs (N-CAFs) and R-CAFs were compared by array analysis. Immortalized human pancreatic CAFs were grown for 30 days in either control media or media containing 100nM gemcitabine. RNA was then isolated and hybidized on U133 Plus 2.0 Affymetrix arrays.

2016-03-09 | E-GEOD-78982 | biostudies-arrayexpress

ATF4 governs functional expansion of hematopoietic stem cells partially via Angptl3 in the fetal liver microenvironment (array)

Project description:In this study, we demonstrated that deletion of the activating transcription factor 4 (ATF4) resulted in severely impaired HSC expansion in the fetal liver at E12.5 and E15.5. In contrast, generation of the first HSC population in the aorta-gonad-mesonephros region at E11.5 was not significantly affected. Furthermore, the HSC-supporting ability of both endothelial and stromal cells in fetal liver was significantly compromised in the absence of ATF4. Gene profiling using RNA-seq revealed down-regulated expression of a panel of cytokines in ATF4-/- stromal cells, including angiopoietin-like protein 3 (Angptl3) and vascular endothelial growth factor-A (VEGFA). To investigate the molecular pathways of ATF4 in the stromal cells and LSK cells in the fetal liver.

2015-11-20 | E-GEOD-66381 | biostudies-arrayexpress

Comparison of gene expression profiling of CAFs before and after chemotherapy in human breast cancer microenvironment

Project description:We investigated the chemotherapy-induced response of primary-cultured CAFs and compared its gene expression profiling before and after chemotherapy treatment.

2014-06-27 | E-MTAB-1614 | biostudies-arrayexpress

ATF4 governs functional expansion of hematopoietic stem cells partially via Angptl3 in the fetal liver microenvironment (RNA-Seq)

2015-11-20 | E-GEOD-66380 | biostudies-arrayexpress

Effect of BMP2 on B16F10 melanoma cells

Project description:BMP2 treatment significantly reduces growth of B16F10 melanoma spheroids. This experiment analyses the transcriptome changes in B16F10 cells treated with or without BMP2.

2019-01-01 | GSE117074 | GEO

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