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The ZSWIM8 ubiquitin ligase mediates target-directed microRNA degradation

ABSTRACT: The ZSWIM8 ubiquitin ligase mediates target-directed microRNA degradation

PROVIDER: PRJNA672920 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12924587.fastq.gz	Fastqsanger.gz
	SRR12924588.fastq.gz	Fastqsanger.gz
	SRR12924589.fastq.gz	Fastqsanger.gz
	SRR12924590.fastq.gz	Fastqsanger.gz
	SRR12924591.fastq.gz	Fastqsanger.gz

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The ZSWIM8 ubiquitin ligase mediates target-directed microRNA degradation [dataset3]

Project description:MicroRNAs (miRNAs) associate with Argonaute (AGO) proteins to direct widespread post-transcriptional gene repression. Although association with AGO typically protects miRNAs from nucleases, extensive pairing to some unusual target RNAs can trigger miRNA degradation. Here we found that this target-directed miRNA degradation (TDMD) required the ZSWIM8 Cullin-RING E3 ubiquitin ligase. This and other findings suggested and supported a mechanistic model of TDMD in which target-directed proteolysis of AGO by the ubiquitin–proteasome pathway exposes the miRNA for degradation. Moreover, loss-of-function studies indicated that the ZSWIM8 Cullin-RING ligase accelerates degradation of numerous miRNAs in cells of mammals, flies, and nematodes, thereby specifying the half-lives of most short-lived miRNAs. These results elucidate the mechanism of TDMD and expand the inferred role of TDMD in shaping miRNA levels in bilaterian animals.

2020-12-18 | GSE163388 | GEO