Project description:A toxicogenomic analysis from liver of different pharmacological active coumarins (mammea A/BA+A/BB 3:1 and soulatrolide ) was performed on mice treated (20mg/kg/daily) for a whole week to evaluate if such compounds possess or could develop a hazardous profile on liver. In this dataset, we include the expression data obtained from dissected liver mouse treated with active coumarins (obtained from Calophyllum brasiliense) and control (SSI) for a whole week. Livers from 4 animals per treatment and control were subjected to RNA extraction. These data showed 46 genes up and 72 downregulated genes for mammea coumarins and 665 up and 1077 downregulated genes . Expression data from liver of mice treated with different coumarins (mammea and soulatrolide) from Calophyllum brasiliense 12 Total samples were analyzed. Liver from 4 animals per treatment groups were subjected to mRNA expression analysis. We generated the statistical computing and metrics in the arrayQualityMetrics data were normalized with RMA method and differential expressed genes obtained with Ingenuity Software. Genes with an FDRâ¤10% and a fold-change â¥2 were selected.
Project description:Here, we adapted and improved our FASTBAC-Seq method originally designed in Helicobacter pylori to investigate T1TAs in the model organism Escherichia coli. Our approach combines a life and death selection with deep-sequencing to assess the killing capability of a toxin and obtain an overview of single-nucleotide substitutions suppressing the toxin expression or activity in absence of its antitoxin. As a proof of concept, we revisited the regulation of the plasmidic hok/Sok T1TA system.
