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Next Generation Sequencing of control and knockout erythroblasts transcriptomes

ABSTRACT: Next Generation Sequencing of control and knockout erythroblasts transcriptomes

PROVIDER: PRJNA674590 | ENA |

REPOSITORIES: ENA

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Next Generation Sequencing of control and knockout erythroblasts transcriptomes

Project description:Purpose: Use next generation sequencing to analyze the cellular pathway change in erythroblasts upon Drosha deletion

2020-11-05 | GSE160820 | GEO

Next generation sequencing following CUX1 knockdown in erythroblasts

Project description:Next generation sequencing following CUX1 knockdown in erythroblasts

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Next Generation Sequencing Facilitates Quantitative Analysis of Wild type and RBM3 knockout cortex Transcriptomes during maternal hypothermia

Project description:Next-generation sequencing (NGS) has revolutionized systems-based analysis of gene expression. The goals of this study are to compare the different transcripts between Wild type and RBM3 knockout cortex Transcriptomes during maternal hypothermia

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Next generation sequencing following CUX1 knockdown in erythroblasts

Project description:Anemia is a significant cause of morbidity and mortality in myeloid malignances. Cytogenetic changes are recurrent within malignant hematopoietic stem and progenitor cells, yet their role in anemia pathogenesis remains unknown. One recurrent karyotypic abnormality in myeloid neoplasms is the deletion of part or all of chromosome 7 [-7/del(7q)], which harbors the transcription factor and tumor suppressor gene, CUX1. CUX1 knockdown mouse models develop myeloid disease similar to that seen in humans, including a spontaneous, cell intrinsic, and lethal anemia that develops with age. Here, we elucidate the cellular and molecular mechanisms by which CUX1 regulates erythropoiesis. We demonstrate CUX1 knockdown mice have an aberrant stress erythropoiesis response and decreased survival after acute anemia induction. CUX1 insufficient erythroblasts undergo accelerated cell cycling and increased apoptosis. In line with these phenotypes, transcriptome profiling indicates that CUX1-knockdown elicits increased proliferation and decreased erythroid differentiation gene signatures. ATAC-sequencing demonstrated dramatic, global chromatin opening in CUX1-knockdown erythroblasts. As measured by fluorescence lifetime imaging, this increased chromatin accessibility is concomitant with a disruption in nuclear condensation, which is normally requisite in mammalian erythroblasts for nuclear eviction and terminal differentiation. Finally, we show that CUX1 mediates chromatin compaction by promoting histone deacetylation. Thus, rather than a transcriptional role, our data implicate in an epigenetic regulatory role for CUX1 in erythropoiesis. Furthermore, these results suggest therapeutic targeting of epigenetic regulators, such as histone acetyl transferases, may have clinical benefit for the anemias associated with loss of CUX1.

2021-08-10 | GSE181626 | GEO

Erythroblasts

Project description:Erythroblasts

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Next Generation Sequencing Facilitates Quantitative Analysis of young nand aged control and Acsl6-/- Transcriptomes

Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare NGS-derived cerebellar transcriptome profiling (RNA-seq) to determine age-relatined impact of acsl6 deficiency on brain transcriptome profiles. Methods: Cerebellar mRNA profiles of 2-month and 18-month control and long-chain acyl-coa synthetase 6-knockout (Acsl6) mice were generated and sent to Genewiz for RNAseq analysis Results: Using an optimized data analysis workflow, we mapped about 3,451,694,754 sequence reads total and ~25-45 million reads per sample of which ~98% were mapped to the mouse genome. Conclusions: Our study represents the first detailed analysis of cerebellar transcriptomes of Acsl6 knockout mice, compated to controls, at young and old ages.

2021-05-27 | GSE175571 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of PHB-knockdown and PHB-knockout Spermatocytes Transcriptomes

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of PHB-knockdown and PHB-knockout Spermatocytes Transcriptomes

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Next-Generation Sequencing Facilitates Quantitative Analysis of Wild Type and DAZAP1 Knockout of ESCC Transcriptomes

Project description:Next-Generation Sequencing Facilitates Quantitative Analysis of Wild Type and DAZAP1 Knockout of ESCC Transcriptomes

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Next Generation Sequencing Facilitates Quantitative Analysis of Transcriptomes of Skeletal Muscle-Specific Akt Knockout Mice

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of Transcriptomes of Skeletal Muscle-Specific Akt Knockout Mice

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Next Generation Sequencing of different ovine muscle's Transcriptomes

Project description:We report the application of single-molecule-based sequencing technology for high-throughput profiling of different ovine muscle's transcriptomes. 9.27 gigabases of sequence from two different breeds of sheep. Examination of 2 different muscle's transcriptomes in 2 breed types.

2013-12-01 | E-GEOD-43316 | biostudies-arrayexpress

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