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Gallus gallus

ABSTRACT: RNAseq sequence reads of spleen of chickens infected with Newcastle disease virus

PROVIDER: PRJNA675698 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13021942_1.fastq.gz	Fastqsanger.gz
	SRR13021942_2.fastq.gz	Fastqsanger.gz
	SRR13021943_1.fastq.gz	Fastqsanger.gz
	SRR13021943_2.fastq.gz	Fastqsanger.gz
	SRR13021944_1.fastq.gz	Fastqsanger.gz

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Expression data of spleens from chickens infected with different Newcastle Disease Viruses

Project description:Genotype VIId NDV is characterized by severe tissue damage in chicken lymphoid organs compared to other virulent strains. However, biological basis of this unusual pathological phenotype is unknown. Host response is associated with pathogenicity of Newcastle Disease Virus (NDV). We aim to determine the contribution of host response to the severe tissue destruction in the lymphoid system caused by genotype VIId NDV. We used microarray analysis to evaluate the global transcriptional response in the spleen of chickens infected with genotype VIId NDV strain JS5/05 and genotype IV NDV Herts/33. Chickens were inoculated with JS5/05 or Herts/33 or mock-infected. At day 2 post infection, spleens were isolated from three chickens per group for RNA extraction and hybridization on Affymetrix microarrays. Samples were named as follows: JS5/05 (I4_1_NS,I4_2_NS,I4_3_NS), Herts/33 (H1_NS,H2_NS, H3_NS), control (C1_NS, C2_NS, C3_NS).

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Project description:Expression data of spleens from chickens infected with different Newcastle Disease Viruses

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Project description:Relative expression levels of mRNAs in chicken cecal epithelia experimentally infected with Eimeria tenella were measured at 4.5 days post-infection. Two weeks old chickens were uninfected (negative control) or were orally inoculated with sporulated oocysts of Eimeria tenella. Cecal epithelia samples were collected from >12 birds in infected or uninfected group at 4.5 d following infections, in which samples from 4 birds were pooled together to form a total 3 biological replicates in each group. Parasite merozoites were also collected from four infected chickens at 5 d after infections. Uninfected control samples, merozoites and infection group samples were selected for RNA extraction and hybridization on Affymetrix microarrays. We used Affymetrix GeneChip chicken genome arrays to detail the chicken cecal epithelia gene expression in the control and E. tenella-infected birds.

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