Project description:synchronized WT_SK1 meiotic time course The SK1 strain background undergoes meiosis with high efficiency and synchronicity (ATCC:MYA-2089)
Project description:Gene expression of human knockouts of telomere-protein Rap1 were compared to their wildtype counterparts in mutliple cell lines (available from the ATCC repository). We discovered that Rap1, desite being mostly associated with the telomere, performed a conserved role in transcriptional regulation, as evidenced by a number of genes that were differentially regulated upon deletion of Rap1.
Project description:Gene expression of human knockouts of telomere-protein Rap1 were compared to their wildtype counterparts in mutliple cell lines (available from the ATCC repository). We discovered that Rap1, desite being mostly associated with the telomere, performed a conserved role in transcriptional regulation, as evidenced by a number of genes that were differentially regulated upon deletion of Rap1. Total RNA was obtained from multiple knockout and wildtype clones generated in three different human parental cell lines obtained from ATCC. Datasets of multiple wild type and knockout clones were compared within cell lines, and not across cell lines, as differences in origin of cell lines led to extremely different transcriptional profiles.
Project description:Synchronized mer1D_SK1 meiotic time course. mer1D diploid was generated in the SK1 strain background (ATCC: MYA-2089) by PCR-mediated cassette-based gene replacement where the MER1 ORF was replaced with HIS3 from S. kluyveri then the transformants were sporulated and the homozygous deletion was verified by PCR.
