Project description:This study investigates the impact of Dexamethasone on sperm RNA in mice. Using RNA sequencing we find that Dexamethasone impacts the sperm payload significantly 2 weeks post intra peritoneal administration. Sperm RNA was sequenced 14 days following 2mg/kg dexamethasone or vehicle intraperitoneal injection.
Project description:2-cell embryo RNA was sequenced following IVF of naive untreated oocytes with sperm harvested 2 weeks after 2mg/kg dexamethasone or vehicle intraperitoneal injection in males.
Project description:Sixteen male Sprague-Dawley rats were randomly allocated into 2 groups (8 rats per group) as follows: the control group (CON) and the dexamethasone-treated group (DEXA). Dexamethasone-treated rats received a daily intraperitoneal injection of 1.5 mg/kg of dexamethasone for 5 days. All rats were fasted during the night following the fifth day. On the sixth day, the animals were killed by decapitation. In order to focus our investigation on metabolism-related genes, we developed a metabolism dedicated microarray tool: the Mitoligo. Using this microarray tool, we were able to determine that energy metabolism was deeply modified by dexamethasone treatment. Dexamethasone treatment to rats induces a complete switch of the metabolism toward a maximal rate of ATP synthesis. In this study, we show that substrate supplying for oxidative phosphorylation is greatly enhanced. We also confirm that oxidative phosphorylation capacity is increased by dexamethasone treatment. Keywords: hormonal treatment