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The chaperonin GroESL supports the functions of the Z-ring regulators FtsA and FzlA

ABSTRACT: The chaperonin GroESL supports the functions of the Z-ring regulators FtsA and FzlA

PROVIDER: PRJNA681511 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13165832.fastq.gz	Fastqsanger.gz
	SRR13165833.fastq.gz	Fastqsanger.gz

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The chaperonin GroESL supports the functions of the Z-ring regulators FtsA and FzlA

Project description:The highly conserved chaperonin GroESL performs a crucial role in protein folding, however the essential cellular pathways that rely on this chaperone are underexplored. Loss of GroESL leads to severe septation defects in diverse bacteria, suggesting the folding function of GroESL may be integrated with the bacterial cell cycle at the point of cell division. Here, we describe new connections between GroESL and the bacterial cell cycle using the model organism Caulobacter crescentus. Using a proteomics approach, we identify candidate GroESL client proteins that become insoluble or are degraded specifically when GroESL folding is insufficient, revealing several essential proteins that participate in cell division and peptidoglycan biosynthesis. We demonstrate that other cell cycle events such as DNA replication and chromosome segregation are able to continue when GroESL folding is insufficient. We further find that deficiency of two FtsZ-interacting proteins, the bacterial actin homologue FtsA and the constriction regulator FzlA, mediate the GroESL-dependent block in cell division. Our data show that sufficient GroESL is required to maintain normal dynamics of the FtsZ scaffold and divisome functionality in C. crescentus. In addition to supporting divisome function, we show that GroESL is required to maintain the flow of peptidoglycan precursors into the growing cell wall. Linking a chaperone to cell division may be a conserved way to coordinate environmental and internal cues that signal when it is safe to divide.

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