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RNAsequencing of control and STAT3 knocked down transriptomes of EndoC cells

ABSTRACT: RNAsequencing of control and STAT3 knocked down transriptomes of EndoC cells

PROVIDER: PRJNA683224 | ENA |

REPOSITORIES: ENA

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RNAsequencing of control and STAT3 knocked down transriptomes of EndoC cells

Project description:Most obese and insulin resistant individuals do not develop diabetes. This is the result of the capacity of β-cells to adapt and produce enough insulin to cover the needs of the organism. The underlying mechanism of β-cell adaptation in obesity, however, remains unclear. Previous studies have suggested a role for STAT3 in mediating β-cell development and human glucose homeostasis, but little is known about its role in β-cells in obesity. We observed enhanced cytoplasmatic expression of STAT3 in severe obese and diabetic subjects. To address the functional role of STAT3 in adult β-cells, we generated mice with tamoxifen-inducible partial or full deletion of STAT3 in β-cells and fed them a high fat diet before analysis. Interestingly, β-cell homozygous and heterozygous STAT3 deficient obese mice showed glucose intolerance when compared to controls. Gene expression analysis by RNA-seq showed reduced expression of mitochondrial genes in STAT3 knocked-down human EndoC-βH1 cells and was confirmed in FACS-purified β-cells from STAT3 deficient mice. Moreover, knockdown of STAT3 impaired mitochondria activity in EndoC-βH1 and human islets, suggesting a mechanism for STAT3-regulated β-cell function. We propose non-canonical STAT3 activity as a marker of β-cell identity, improving glucose induced insulin secretion in obesity.

2021-11-15 | GSE162837 | GEO

Gene expression by TRPS1 knocked down in T47D

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| PRJNA408130 | ENA

Gene expression by LASP1 knocked down in PC3 cells

Project description:Gene expression by LASP1 knocked down in PC3 cells

| PRJNA655418 | ENA

Gene expression by BTF3 knocked down in Vcap cells

Project description:Gene expression by BTF3 knocked down in Vcap cells

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Expression data from SIX1 knocked-down HKc/DR cells

Project description:Expression data from SIX1 knocked-down HKc/DR cells

| PRJNA523785 | ENA

RNA sequencing of control and PTPN2 knocked down transcriptomes in EndoC-훽H1 cells with or without the treatment of pro-inflammatory cytokines

Project description:RNA sequencing of control and PTPN2 knocked down transcriptomes in EndoC-훽H1 cells with or without the treatment of pro-inflammatory cytokines

| PRJNA722250 | ENA

The gene expression analysis of TDP knocked down MIN6 cells.

Project description:The gene expression analysis of TDP knocked down MIN6 cells.

| PRJNA516346 | ENA

RNA-seq analysis about Hira/Ubn1/Ubn2 knocked down samples

Project description:RNA-seq analysis about Hira/Ubn1/Ubn2 knocked down samples

| PRJNA736849 | ENA

Gene expression of S100A10 knocked down human breast cancer cells

Project description:Gene expression of S100A10 knocked down human breast cancer cells

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Proteomic analysis of Nrf2 knocked down in HT29

Project description:Colorectal cancer is known as one of the most common cancer and the third leading cause of death worldwide. Nrf2 is considered as a main transcription factor for producing antioxidants in response to oxidative stress and reduce damages to the cell. It also plays a crucial role in drug resistance. In this study, the Nrf2 gene was knocked down in HT-29 cell line and then the protein expression profile were investigated by TMT based LC MS/MS approach.

2022-05-04 | PXD027360 | Pride

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