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MRNA expression profiles of murine macrophages (cell line: RAW264.7)

ABSTRACT: mRNA expression profiles of murine macrophages (cell line: RAW264.7)

PROVIDER: PRJNA684304 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13318073_1.fastq.gz	Fastqsanger.gz
	SRR13318073_2.fastq.gz	Fastqsanger.gz
	SRR13318074_1.fastq.gz	Fastqsanger.gz
	SRR13318074_2.fastq.gz	Fastqsanger.gz
	SRR13318075_1.fastq.gz	Fastqsanger.gz

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Project description:miRNA expression profiles of murine macrophages (cell line: RAW264.7)

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Project description:mRNA and miRNA expression profiles of murine macrophages (cell line: RAW264.7)

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miRNA expression profiles of murine macrophages (cell line: RAW264.7)

Project description:We report the miRNA profile of murine macrophages (cell line: RAW264.7) after supplementation with polyunsaturated fatty acids (PUFA) and stimulation with LTA. The fatty acids docosahexaenoic acid (DHA, C22:6n3) or arachidonic acid (AA, C20:4n6) were included in the culture medium in concentrations of 15 µmol/L using ethanol as a vehicle (0.2 % v/v final ethanol concentration). Cells were cultured in the enriched media totaling 72 h. Stimulation of cells was performed in the last 24 h of fatty acid supplementation by addition of LTA (0.5 µg/mL; from Staphylococcus aureus).

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Expression data from murine RAW264.7 macrophages

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miRNA expression profiles of macrophages (cell line: RAW264.7) and endothelial cells (cell line: TIME)

Project description:We report the miRNA profiles of macrophages (cell line: RAW264.7) and endothelial cells (cell line: TIME) after supplementation with polyunsaturated fatty acids (PUFA) and stimulation with LPS/pro-inflammatory cytokines. The fatty acids docosahexaenoic acid (DHA, C22:6n3) or arachidonic acid (AA, C20:4n6) were included in the culture medium in concentrations of 15 µmol/L using ethanol as a vehicle (0.2 % v/v final ethanol concentration). Cells were cultured in the enriched media totaling either 72 h (RAW264.7) or 144 h (TIME). Stimulation of cells was performed in the last 24 h of fatty acid supplementation by addition of either LPS (1 µg/mL; from E. coli serotype 0111:B4) for cell line RAW264.7 or the cytokines IL-1β, TNF-α, and IFN-γ each in a concentration of 5 ng/ml for cell line TIME.

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Expression data from RAW264.7 murine cell line

Project description:Expression data from RAW264.7 murine cell line

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Effect of C2S in RAW264.7 murine macrophages

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