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H3K27me3 maintains the transcription program during epigenetic reprogramming in the mouse germ line [RNA-seq D4]

ABSTRACT: H3K27me3 maintains the transcription program during epigenetic reprogramming in the mouse germ line [RNA-seq D4]

PROVIDER: PRJNA685846 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13263451_1.fastq.gz	Fastqsanger.gz
	SRR13263451_2.fastq.gz	Fastqsanger.gz
	SRR13263452_1.fastq.gz	Fastqsanger.gz
	SRR13263452_2.fastq.gz	Fastqsanger.gz
	SRR13263453_1.fastq.gz	Fastqsanger.gz

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H3K27me3 maintains the transcription program during epigenetic reprogramming in the mouse germ line

Project description:H3K27me3 maintains the transcription program during epigenetic reprogramming in the mouse germ line

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H3K27me3 maintains the transcription program during epigenetic reprogramming in the mouse germ line [RNA-seq D4]

Project description:To investigate the role of EZH2 in transcription regulation in mouse primordial germ cell like cells (PGCLCs), we isolated Ctrl and Ezh2 KO PGCLCs. Our data demonstrates that EZH2-mediated H3K27me3 is dispensible for transcription regulation in PGCLCs.

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H3K27me3 maintains the transcription program during epigenetic reprogramming in the mouse germ line [ChIP-seq]

Project description:H3K27me3 maintains the transcription program during epigenetic reprogramming in the mouse germ line [ChIP-seq]

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H3K27me3 maintains the transcription program during epigenetic reprogramming in the mouse germ line [RNA-seq]

Project description:H3K27me3 maintains the transcription program during epigenetic reprogramming in the mouse germ line [RNA-seq]

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H3K27me3 maintains the transcription program during epigenetic reprogramming in the mouse germ line [RNA-seq]

Project description:To investigate the role of EZH2 in transcription regulation in mouse primordial germ cells, we isolated Ctrl and Ezh2 CKO male and female PGCs at E13.5. Our data demonstrates that EZH2-mediated H3K27me3 is required for regulating the expression of meiotic genes in the female germ cells. Furthermore, RNA-seq analysis shows that EZH2 is essential for transcriptional repression of retrotransposons.

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H3K27me3 maintains the transcription program during epigenetic reprogramming in the mouse germ line [ChIP-seq]

Project description:We used Ultra-Low native ChIP to interrogate the H3K27me3 and H3K9me3 chromatin states in primordial germ cells before and after gonadal reprogramming. Our data demonstrate that repressive heterochromatin is remodelled during epigenetic reprogramming.

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CUT&RUN against H3K27ac and H3K27me3 on WT and TKO ESCs (D0), and D2, D4 EpiLCs

Project description:We investigate the impact of DNA methylation on chromatin epigenetic marks during epiblast differentiation. To this end, we used in vitro Epiblast-like cell (EpiLC) differentiation on WT and DNA-methylation free TKO murine ESCs. We next performed CUT&RUN against H3K27me3 and H3K27ac at D0, D2 and D4.

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Project description:In many metazoans, germ cells are separated from somatic lineages early in development and maintain their identity throughout life. Here we show that a Polycomb group (PcG) component, Enhancer of Zeste [E(z)] H3K27me3-specific methyltransferase, maintains germline identity in Drosophila adult testes. We find excessive early-stage somatic gonadal cells in E(z) mutant testes, which originate from both over-proliferative cyst stem cells and germ cells turning on an early-stage somatic cell marker. Using complementary lineage-tracing experiments in E(z) mutant testes, a portion of excessive early-stage somatic gonadal cells are found to derive from early-stage germ cells, including germline stem cells. Interestingly, knocking down E(z) specifically in somatic cells caused this germline-to-soma change, suggesting a non-cell autonomous role of E(z) to antagonize somatic identity in germ cells. Using fly testis specifically expressing E(z) shmiR RNAi in germ cells by nos promoter driven GAL4>UAS system, ChIPseq with H3K27me3 antibody was performed, where H3K27me3 is only detected in somatic cells.

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