Similar Datasets
Project description:Transcriptional profiling of mosquito head comparing control non-swarming mosquito with swarming mosquito. Screen the genes associated with swarming.
2020-09-19 | GSE150971 | GEO
Project description:Aedes aegypti are vectors of several devastating arboviruses infecting hundreds of millions of people annually. Controlling mosquito populations by regulating their reproduction is important to minimize viral transmission in the absence of effective antiviral therapies or vaccines. Here, we demonstrate that leucine aminopeptidase1 (LAP1), screened from SWATH-MS-based proteomic data of female spermathecae, is a crucial determinant in mosquito population expansion. Mitochondrial defects and aberrant autophagy of sperm in LAP1 mutant males (LAP1-/-), prepared using CRISPR-Cas9 system, resulted in a reduction of reproduction in wild-type females that mated with them. Additionally, we found that the fitness of LAP1-/- males was strong enough to efficiently transmit genetic changes to mosquito populations through a low number of hatchable offspring, making it to be a promising opportunity to suppress mosquito populations using LAP1-/- males. Importantly, we provide a novel target gene for genetic drive, further amplifying the function of LAP1 in reducing mosquito populations.
2023-02-05 | PXD039869 | iProX
Project description:We have published a paper showing that infections using mosquito-transmitted parasites have a quite different effect on the host immune system (Spence et al, Nature, 2013). Here we are repeating the experiment but using a more virulent strain of the parasite: P. chabaudi CB, to study further how mosquito transmission regulates parasite virulence. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
2014-08-27 | E-ERAD-289 | biostudies-arrayexpress
Project description:Anopheles gambiae mosquitoes are primary human malaria vectors, but we know very little about their mechanisms of transcriptional regulation. We profiled chromatin accessibility by ATAC-seq in laboratory-reared An. gambiae mosquitoes experimentally infected with the human malaria parasite Plasmodium falciparum. By integrating ATAC-seq, RNA-seq and ChIP-seq data we showed a positive correlation between accessibility at promoters and introns, gene expression and active histone marks. By comparing expression and chromatin structure patterns in different tissues, we were able to infer cis-regulatory elements controlling tissue specific gene expression and to predict the in vivo binding sites of relevant transcription factors. The ATAC-seq assay also allowed the precise mapping of active regulatory regions, including novel transcription start sites and enhancers that annotate to mosquito immune-response genes. This study is important not only for advancing our understanding of mechanisms of transcriptional regulation in the mosquito vector of human malaria, but also the information we produced is of great potential for developing new mosquito-control and anti-malaria strategies.
2020-12-22 | GSE152924 | GEO
Project description:Kinetoplastids are a group of parasite species, several of which cause important diseases in human and livestock. Nearly all of these pathogenic species are transmitted by insect vectors, in which the parasites undergo a specific developmental program. One shared event undergone by multiple species is adherence to insect tissue. This adhesion occurs by means of a hemidesmosome-like structure that is thus far uncharacterized. We have used the monoxenous parasite Crithidia fasciculata, which exclusively infects mosquitoes, to study this process of parasite adhesion in the insect. We have transcriptionally profiled adherent and swimming forms of the parasite that have been generated in vitro, and compared these profiles to the adhesive form in the mosquito. Using a dual-RNAseq approach, we have also identified several genes that are differentially regulated in infected versus uninfected mosquitoes, including several immune genes. This indicates that the mosquito is responding to the presence of the parasites.
2019-06-26 | GSE132641 | GEO
Project description:To test the contributions of phagocytic immune cells to mosquito immunity, we depleted mosquito phagocytes using clodronate liposomes and then challenged mosquitoes with malaria parasites. Observing a large increase in infection intensity, the purpose of the RNA-seq experiment is to determine their effects of phagocyte depletion on mosquito immunity.
2020-04-10 | GSE116156 | GEO
Project description:MalariaM-bM-^@M-^Ys cycle of infection requires parasite transmission between a mosquito vector and a vertebrate host. Plasmodium regulates transmission by translationally repressing specific mRNAs until their products are needed. We demonstrate that the Plasmodium yoelii Pumilio-FBF family member Puf2 allows the sporozoite to retain its infectivity in the mosquito salivary glands while awaiting transmission. Puf2 mediates this critical feature solely through its RNA-Binding Domain (RBD) likely by protecting and silencing specific mRNAs. Puf2 storage granules are distinct from stress granules and P-bodies and dissolve rapidly after infection of hepatocytes, likely releasing the protected and silenced transcripts for M-bM-^@M-^Xjust-in-timeM-bM-^@M-^Y translation by early exoerythrocytic forms (EEFs). Further corroborating this model, pypuf2- sporozoites have no apparent defect in host infection early after invading the salivary glands, but become progressively noninfectious and subsequently prematurely transform into EEFs during prolonged salivary gland residence. In contrast, the premature overexpression of Puf2 in oocysts causes striking deregulation of sporozoite maturation, resulting in fewer oocyst sporozoites that are non-infectious and unable to colonize the salivary glands. Maintenance of Puf2 expression in liver stage parasites produces no phenotype, suggesting that a window of permissive expression exists. Finally, by conducting the first comparative RNAseq analysis of Plasmodium sporozoites, we have uncovered that Puf2 may play a role in both the protection of specific transcripts as well as RNA turnover via the CCR4/Not complex. These findings uncover requirements for maintaining a window of opportunity for the malaria parasite to accommodate the unpredictable moment of transmission from mosquito to vertebrate host. Wild-type (Py17XNL) and pypuf2 -salivary gland sporozoites
2012-10-27 | E-GEOD-41873 | biostudies-arrayexpress