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Puccinia triticina

ABSTRACT: Puccinia triticina Transcriptome sequencing and assembly

PROVIDER: PRJNA694214 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13577170_1.fastq.gz	Fastqsanger.gz
	SRR13577170_2.fastq.gz	Fastqsanger.gz
	SRR13577171_1.fastq.gz	Fastqsanger.gz
	SRR13577171_2.fastq.gz	Fastqsanger.gz
	SRR13577172_1.fastq.gz	Fastqsanger.gz

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Inoculation of rice with the non-host pathogen Puccinia triticina f. sp. tritici elicits an active transcriptional response

Project description:Japonica rice cultivar Nipponbare was inoculated with wheat leaf rust (Puccinia triticina f. sp. tritici, non-host pathogen to rice) to compare gene expression profiles with mock-inoculated controls. Although eventually failed in invasion, leaf rust induced a set of rice genes that were distinctally up-regulated, some of those were confirmed by quantitative real-time PCR assays.

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Project description:In this experiment the transcriptome reprogramming in wheat during host and nonhost interaction with Puccinia sp. was analyzed in a time-series approach. Ten days old wheat plants of cv. Renan were mock-inoculated or inoculated with P. triticina (Pt), BRW96258 isolate, or P. hordei (Ph), 1.2.1 isolate. After 12, 24, 36 and 48 hours first leaves were sampled. Total RNA was extracted using the RNeasy Plant Mini Kit (Qiagen, Hilden, Germany), the Ambion TURBO DNA-free DNase Kit was used for DNA elimination, and RNA was hybridized to Agilent 44k oligonucleotide arrays.

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Transcription profiling by array of barley after inoculation with Puccinia hordei, Puccinia triticina or mock-inoculation to identify candidate genes for nonhost resistance against rust disease

Project description:In this experiment the transcriptome reprogramming in barley during host and nonhost interaction with Puccinia sp. was analyzed in a time-series approach. Ten days old barley plants of cv. Vada were mock-inoculated or inoculated with P. hordei (Ph), 1.2.1 isolate, or P. triticina (Pt), BRW96258 isolate. After 12, 24, 36 and 48 hours first leaves were sampled. Total RNA was extracted using the RNeasy Plant Mini Kit (Qiagen, Hilden, Germany), the Ambion TURBO DNA-free DNase Kit was used for DNA elimination, and RNA was hybridized to Agilent 44k oligonucleotide arrays.

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