Project description:Tumor heterogeneity of high-grade glioma (HGG) is recognized by four clinically relevant subtypes based on core gene signatures. However, molecular signaling in glioma stem cells (GSCs) in individual HGG subtypes is poorly characterized. Here we identified and characterized two mutually exclusive GSC subtypes with distinct dysregulated signaling pathways. Analysis of mRNA profiles distinguished proneural (PN) from mesenchymal (Mes) GSCs and revealed a pronounced correlation with the corresponding PN or Mes HGGs. Mes GSCs displayed more aggressive phenotypes in vitro and as intracranial xenografts in mice. Further, Mes GSCs were markedly resistant to radiation compared with PN GSCs. The glycolytic pathway, comprising aldehyde dehydrogenase (ALDH) family genes and in particular ALDH1A3, were enriched in Mes GSCs. Glycolytic activity and ALDH activity were significantly elevated in Mes GSCs but not in PN GSCs. Expression of ALDH1A3 was also increased in clinical HGG compared with low-grade glioma or normal brain tissue. Moreover, inhibition of ALDH1A3 attenuated the growth of Mes but not PN GSCs. Last, radiation treatment of PN GSCs up-regulated Mes-associated markers and downregulated PN-associated markers, whereas inhibition of ALDH1A3 attenuated an irradiation-induced gain of Mes identity in PN GSCs. Taken together, our data suggest that two subtypes of GSCs, harboring distinct metabolic signaling pathways, represent intertumoral glioma heterogeneity and highlight previously unidentified roles of ALDH1A3-associated signaling that promotes aberrant proliferation of Mes HGGs and GSCs. Inhibition of ALDH1A3- mediated pathways therefore might provide a promising therapeutic approach for a subset of HGGs with the Mes signature.

2015-03-20 | GSE67089 | GEO

Ferrovum sp. PN-J185

Project description:Ferrovum sp. PN-J185 Genome sequencing and assembly

| PRJNA305480 | ENA

Project description:Microbial community of a mainstream PN/A reactor

| PRJNA820722 | ENA

Parthenolide induces ROS-dependent cell death in human gastric cancer cells

Project description:Parthenolide (PN), a key active ingredient of feverfew, has been used to treat gastrointestinal disorders. However, the mechanism of the cytotoxic effect exerted by PN on tumor cells is not elucidated now. MGC-803 cells treated with and without PN were separately subjected to high throughput RNA sequencing (RNA-Seq).

2023-12-31 | GSE217526 | GEO

Sandaracinobacteroides hominis strain:SZY PN-1

Project description:Sandaracinobacteroides hominis strain:SZY PN-1 Genome sequencing and assembly

| PRJNA669492 | ENA

anammox and denitrification in mainstream MBBR

Project description:anammox and denitrification in mainstream MBBR Raw sequence reads

| PRJNA765196 | ENA

partial denitrification and anammox in mainstream MBBR

Project description:partial denitrification and anammox in mainstream MBBR Raw sequence reads

| PRJNA765198 | ENA