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Targeting pH-responsive Transcriptional Regulation of Peptidoglycan Cleavage Potentiates Fast Clearance of Mycobacterium tuberculosis with Frontline Antibiotics

ABSTRACT: Targeting pH-responsive Transcriptional Regulation of Peptidoglycan Cleavage Potentiates Fast Clearance of Mycobacterium tuberculosis with Frontline Antibiotics

PROVIDER: PRJNA702056 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13529913_1.fastq.gz	Fastqsanger.gz
	SRR13529913_2.fastq.gz	Fastqsanger.gz
	SRR13529914_1.fastq.gz	Fastqsanger.gz
	SRR13529914_2.fastq.gz	Fastqsanger.gz
	SRR13529915_1.fastq.gz	Fastqsanger.gz

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Targeting pH-responsive Transcriptional Regulation of Peptidoglycan Cleavage Potentiates Fast Clearance of Mycobacterium tuberculosis with Frontline Antibiotics

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Expression data from peptidoglycan stimulated CD11b+ cells from NOD and NOD.Idd3

Project description:CD11b+ CD11c- cells were isolated by cell sorting from the spleens of NOD and NOD.Idd3 mice. Cells were then either unstimulated or stimulated with peptidoglycan (5ug/ml) for 4 hours at 37 degrees prior to RNA extraction. Peptidoglycan was chosen as a stimulus due to its know ability to stimulate CD11b+ cells through Toll-like receptor 2 and our previous data showing biological differences between NOD and NOD.Idd3-derived CD11b+CD11c- cells after stimulation with peptidoglycan. CD11b+ cells from NOD and NOD.Idd3 were either unstimulated or stimulated with peptidoglycan(PGN) for 4 hours at 37 degerees prior to RNA extraction.

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