SHARC-seq: High throughput in vivo determination of 3D RNA structures and dynamics
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ABSTRACT:
PROVIDER: PRJNA705199 | ENA |
REPOSITORIES: ENA
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Project description:RNA molecules not only carry genetic information like DNA, but also folds into exquisite 3D structures like proteins. Despite strong interests in RNA biology and their medical applications, RNA structure determination in vivo remained a long-standing problem. Here we developed a new technology to directly determine RNA structures in vivo, termed SHARC-seq. Applying SHARC-seq, spatial distances among nucleotides can be measured and used to rebuild in vivo RNA 3D structure and dynamics.
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