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Influenza A virus strain:A/Puerto Rico/8/1934

ABSTRACT: Influenza A virus defective viral genomes are inefficiently packaged into virions relative to wild-type genomic RNAs.

PROVIDER: PRJNA725907 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14352105_1.fastq.gz	Fastqsanger.gz
	SRR14352105_2.fastq.gz	Fastqsanger.gz
	SRR14352106_1.fastq.gz	Fastqsanger.gz
	SRR14352106_2.fastq.gz	Fastqsanger.gz
	SRR14352107_1.fastq.gz	Fastqsanger.gz

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Small RNA-Seq of influenza A/Puerto Rico/8/1934 (H1N1) virus infected A549 human cells

Project description:To study expression pattern of small nucleolar RNAs (snoRNAs) during influenza A viral infection, human cells A549 were infected with influenza A/Puerto Rico/8/1934 (H1N1) virus. Small RNA-seq analysis of infected cells after 24 h or 48 h incubation was performed on an Illumina NextSeq 500 platform. The same mock-infected cells were used as control. Small RNA fractions (<200 nucleotide length) was used for constructing of cDNA libraries. Differential expressed non-coding RNAs were identified using R package DESeq2.

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Project description:Influenza A virus (A/Puerto Rico/8/1934(H1N1))

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RNA-Seq transcriptome data of human cells infected with influenza A/Puerto Rico/8/1934 (H1N1) virus

Project description:Human influenza remains a serious public health problem. This data article reports the transcriptome analysis data of human cell lines infected with influenza A/Puerto Rico/8/1934 (H1N1) virus. Mock-infected cells were included as controls. Human embryonic fibroblasts (MRC-5) and immortalized cell lines (A549, HEK293FT, WI-38 VA-13) were selected for RNA sequencing using Illumina NextSeq500 platform. Raw data were applied to the bioinformatic pipeline, which includes quality control with FastQC and MultiQC, adapter and quality trimming with Cutadapt, filtering to the genome of influenza A with STAR, transcript quantification with Salmon tool (GRCh38_RefSeq_Transcripts). Differential expressed genes were identified using R package DESeq2 with FDR-adjusted p-value < 0.001 and absolute value of log2(FC) > 1. Lists of differentially expressed genes is provided. The raw and processed RNA-seq data presented in this article were deposited to the European Nucleotide Archive via the ArrayExpress partner repository with the dataset accession number E-MTAB-9511 .

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