Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Marine sediment metagenome

ABSTRACT: San Pedro Basin, California Barrel Dumpsite

PROVIDER: PRJNA726559 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other
	JAHOYU01.dat.gz	Other
	JAHOYU01.fasta.gz	Fasta.gz
	JAHOYU01.master.dat	Other
	JAHOYX01.dat.gz	Other

Items per page:

1 - 5 of 11

Similar Datasets

Identification of Arsenic Resistance Genes from Marine Sediment Metagenome.

Project description:A metagenomic library of sea sediment metagenome containing 245,000 recombinant clones representing ~ 2.45 Gb of sea sediment microbial DNA was constructed. Two unique arsenic resistance clones, A7 and A12, were identified by selection on sodium arsenite containing medium. Clone A7 showed a six-fold higher resistance to arsenate [As(V)], a three-fold higher resistance to arsenite [As(III)] and significantly increased resistance to antimony [Sb(III)], while clone A12 showed increased resistance only to sodium arsenite and not to the other two metalloids. The clones harbored inserts of 8.848 Kb and 6.771 Kb, respectively. Both the clones possess A + T rich nucleotide sequence with similarity to sequences from marine psychrophilic bacteria. Sequence and transposon-mutagenesis based analysis revealed the presence of a putative arsenate reductase (ArsC), a putative arsenite efflux pump (ArsB/ACR) and a putative NADPH-dependent FMN reductase (ArsH) in both the clones and also a putative transcriptional regulatory protein (ArsR) in pA7. The increased resistance of clone A7 to As(V), As(III) and Sb(III) indicates functional expression of ArsC and ArsB proteins from pA7. The absence of increased As(V) resistance in clone A12 may be due to the expression of a possible inactive ArsC, as conserved Arg60 residue in this protein was replaced by Glu60, while the absence of Sb(III) resistance may be due to the presence of an ACR3p-type arsenite pump, which is known to lack antimony transport ability.

| S-EPMC5574776 | biostudies-literature

marine sediment metagenome

Project description:Enriched cells from marine sediment in Santa Monica Basin, California, USA – Roland_Nextera1 metagenome

| PRJNA465948 | ENA

marine sediment metagenome

Project description:Guaymas Basin sediment metagenomic assembly

| PRJNA362212 | ENA

marine sediment metagenome

Project description:Adelie Basin Metagenomes Raw sequence reads

| PRJNA383607 | ENA

marine sediment metagenome

Project description:Metagenomic sequencing from Guaymas Basin Metagenome