Jaw-D;pTCP4::mTCP4:GR_Cycloheximide + Dexamethasone microarray
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA731150 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:9-day-old jaw-D;pTCP4::mTCP4:GR treated with Cycloheximide (CHX, control) and the combination of Cycloheximide and Dexamethasone (CHX DEX, treated).
2021-05-20 | GSE174702 | GEO
Project description:Sixteen male Sprague-Dawley rats were randomly allocated into 2 groups (8 rats per group) as follows: the control group (CON) and the dexamethasone-treated group (DEXA). Dexamethasone-treated rats received a daily intraperitoneal injection of 1.5 mg/kg of dexamethasone for 5 days. All rats were fasted during the night following the fifth day. On the sixth day, the animals were killed by decapitation. In order to focus our investigation on metabolism-related genes, we developed a metabolism dedicated microarray tool: the Mitoligo. Using this microarray tool, we were able to determine that energy metabolism was deeply modified by dexamethasone treatment. Dexamethasone treatment to rats induces a complete switch of the metabolism toward a maximal rate of ATP synthesis. In this study, we show that substrate supplying for oxidative phosphorylation is greatly enhanced. We also confirm that oxidative phosphorylation capacity is increased by dexamethasone treatment. Keywords: hormonal treatment
2008-01-13 | GSE9035 | GEO
Project description:determine genes regulated by dexamethasone in c3h10t1/2 cells after 90 minutes of treatment compared to vehicle Keywords: treatment comparison microarray
2008-03-21 | GSE10900 | GEO
Project description:The rat pancreatic cell line AR42J is relatively undifferentiated under normal culture conditions. When the glucocorticoid dexamethasone is added to the medium the cells display a dramatic decrease in proliferative rate and are induced to a more exocrine phenotype that includes increased expression of exocrine pancreas products (digestive enzymes) and more developed regulated secretion. We used microarray to determine changes in gene expression comparing control (without dexamethasone) vs induced (plus dexamethasone).
2015-09-11 | GSE72907 | GEO
Project description:determine genes regulated by dexamethasone in c3h10t1/2 cells after 90 minutes of treatment compared to vehicle Keywords: treatment comparison microarray c3h10t1/2 cells were treated with vehicle or 1uM dexamethasone for 90 minutes (3 biological replicates of each) RNA samples were isolated from RNeasy kit (Qiagen) hybridized all samples to a pool of RNA from vehicle and treated c3h10t1/2 cells
2010-05-25 | E-GEOD-10900 | biostudies-arrayexpress
Project description:This study investigates the impact of Dexamethasone on sperm RNA in mice. Using RNA sequencing we find that Dexamethasone impacts the sperm payload significantly 2 weeks post intra peritoneal administration. Sperm RNA was sequenced 14 days following 2mg/kg dexamethasone or vehicle intraperitoneal injection.
2020-07-15 | GSE154410 | GEO
Project description:Transgenic plants expressing a microRNA insensitive version of TCP10 (TCP10m) fused to the Glucocortocoid receptor (GR) in the jaw-D background and jaw-D mutants were grown for 21 days on MS agar. Nuclear import of TCP10m-GR was induced by transfer of plantlets to MS medium containing 10M-5M Dexamethasone and 10 M-5M Cycloheximide. The above ground parts of whole plantlets were harvested after 0, 2 and 4 hours.
2013-10-25 | E-TABM-1191 | biostudies-arrayexpress
Project description:Dexamethasone improves the survival of COVID-19 patients in need of supplemental oxygen therapy. Hospitalized COVID-19 patients eligible for dexamethasone therapy were recruited from the general care ward in several centers in Greece and the Netherlands and whole blood transcriptomic analysis was performed before and after starting dexamethasone treatment. Peripheral blood mononuclear cells (PBMCs) were isolated from healthy individuals and COVID-19 patients and stimulated with inactivated SARS-CoV-2 ex vivo in the presence or absence of dexamethasone and their transcriptome was assessed.
2023-07-26 | GSE227585 | GEO
Project description:The rat pancreatic cell line AR42J is relatively undifferentiated under normal culture conditions. When the glucocorticoid dexamethasone is added to the medium the cells display a dramatic decrease in proliferative rate and are induced to a more exocrine phenotype that includes increased expression of exocrine pancreas products (digestive enzymes) and more developed regulated secretion. We used microarray to determine changes in gene expression comparing control (without dexamethasone) vs induced (plus dexamethasone). Three independent pairs of control and dexamethasone-induced cultures were prepared followed by total RNA extraction and microarray analysis using the Affymetrix Rat 230A chip.
2015-09-11 | E-GEOD-72907 | biostudies-arrayexpress
Project description:Sperm RNA was sequenced 14 days following 2mg/kg dexamethasone or vehicle intraperitoneal injection.
2020-11-25 | GSE162110 | GEO