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An ex vivo physiologic and hyperplastic vessel culture model to study intra-arterial stent therapies

ABSTRACT: An ex vivo physiologic and hyperplastic vessel culture model to study intra-arterial stent therapies

PROVIDER: PRJNA731919 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14615882_1.fastq.gz	Fastqsanger.gz
	SRR14615882_2.fastq.gz	Fastqsanger.gz
	SRR14615883_1.fastq.gz	Fastqsanger.gz
	SRR14615883_2.fastq.gz	Fastqsanger.gz
	SRR14615884_1.fastq.gz	Fastqsanger.gz

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An ex vivo physiologic and hyperplastic vessel culture model to study intra-arterial stent therapies

Project description:An ex vivo physiologic and hyperplastic vessel culture model to study intra-arterial stent therapies

| PRJNA731919 | ENA

An ex vivo physiologic and hyperplastic vessel culture model to study intra-arterial stent therapies

Project description:Conventional in vitro methods for biological evaluation of intra-arterial devices such as stents fail to accurately predict cytotoxicity and remodeling events. An ex vivo flow-tunable vascular bioreactor system (VesselBRx), comprising intra- and extra-luminal monitoring capabilities, addresses these limitations. VesselBRx mimics the in vivo physiological, hyperplastic, and cytocompatibility events of absorbable magnesium (Mg)-based stents in ex vivo stent-treated porcine and human coronary arteries, with in-situ and real-time monitoring of local stent degradation effects. Unlike conventional, static cell culture, the VesselBRx perfusion system eliminates unphysiologically high intracellular Mg2+ concentrations and localized O2 consumption resulting from stent degradation. Whereas static stented arteries exhibited only 20.1% cell viability and upregulated apoptosis, necrosis, metallic ion, and hypoxia-related gene signatures, stented arteries in VesselBRx showed almost identical cell viability to in vivo rabbit models (~94.0%). Hyperplastic intimal remodeling developed in unstented arteries subjected to low shear stress, but was inhibited by Mg-based stents in VesselBRx, similarly to in vivo. VesselBRx represents a critical advance from the current static culture standard of testing absorbable vascular implants.

2021-05-22 | GSE174858 | GEO

An ex vivo physiologic and hyperplastic vessel culture model to study intra-arterial stent therapies.

| S-EPMC9195126 | biostudies-literature

The LncRNA promotes vessel co-option mediated antiagiogenic therapies (AATs) resistance

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Stent microbiome

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Real-time quantitative PCR analysis of rat aorta samples after stent deployment

Project description:Bare Metal Stents (BMS), Drug Eluting Stent (DES) and Exosomes Eluting Stent (EES) were placed in rat abdominal aorta for one week. Then total RNA of aortas was extracted. We used ScienCell's GeneQuery Rat Macrophage Polarization Markers qPCR Array Kit to quantitate gene expression of macrophage relevant genes.

2021-04-30 | GSE155793 | GEO

Gene expression of cultured HUVECs submitted to different shear stress in the presence or absence of stent procedure

2013-03-16 | GSE45225 | GEO

Inflammation and Extracellular Matrix Genes Differentially Expressed Following Overlapping Zotarolimus- and Sirolimus-Eluting Stent Implantation

2011-04-22 | GSE28781 | GEO

Gene expression of cultured HUVECs submitted to different shear stress in the presence or absence of stent procedure

Project description:Many studies have characterized the results of shear stress changes on cultured endothelial cells in different bioreactor systems. However it is still unclear how an invasive intervention like stent procedure may influence the transcriptional response of endothelium. To study the simultaneous effects of shear stress changes and stent application on endothelial gene expression, we have used an experimental apparatus of laminar flow bioreactor (LFB) system with human cultured endothelial cells exposed or not exposed to stent procedure with different flow conditions. Microarray analysis was evaluated in each experimental protocol. HUVECs (2nd and 5th passage) covered on Thermanox slides were submitted to static, low and physiological (0, 1, 5 and 10 dyne/cm2) shear stress in absence (AS) or presence (PS) of stent in LFB system for 24h. Affymetryx analysis has been performed in duplicate by Consortium for Genomic Technologies (Cogentech; Milan, Italy)

2013-03-16 | E-GEOD-45225 | biostudies-arrayexpress

Inflammation and Extracellular Matrix Genes Differentially Expressed Following Overlapping Zotarolimus- and Sirolimus-Eluting Stent Implantation

Project description:An unanticipated complication of the use of bare metal stents in percutaneous transluminal coronary angioplasty is in-stent restenosis resulting in >50% late lumen diameter loss in treated patients. In an effort to reduce in-stent restenosis, drug eluting stents containing the immunosuppressant sirolimus or zotarolimus have recently been developed. We report here the molecular response of arterial tissue to the implanting of these drug-eluting stents. Gene expression profiling was performed on 4 artery segments surrounding bare metal stents (BMS), 4 artery segments surrounding sirolimus-eluting stents (SES), and 4 artery segments surrounding zotarolimus-eluting stents (ZES) implanted into porcine animal models for 28 days.

2011-04-22 | E-GEOD-28781 | biostudies-arrayexpress

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