Project description:We performed the long-term administration experiment using a yogurt fermented with Lactobacillus delbrueckii subsp. bulgaricus 2038 and Streptococcus thermophilus 1131 (LB81 yogurt) for 20 months in order to understand the effects of the long-term intake of probiotics on mice. Microarrays were used to compare the gene expressions of the intestine, liver and spleen tissues between control mice and LB81 yogurt-intake mice at 28 months of age. Abbreviations used: A, LB81 yogurt-intake mice; C, control mice
Project description:The aim of the study was to decipher metabolisms responsible (i) for the peculiar adaptation of L. plantarum during soy juice fermentation and (ii) for the release of aroma compounds, amino and short-chain fatty acid, and metabolites with health-promoting properties in soy yogurt. The strategy was the sequencing and annotation of a strain (L. plantarum CIRM-BIA777, PRJEB77707) able to degrade galacto- oligosaccharides, the sampling of soy yogurt, RNA-seq to identify differentially expressed genes of L. plantarum and corresponding metabolisms throughout the kinetics of fermentation. Acids and volatile compounds were also quantified.
Project description:Intervention 1: Intervention group: Symptomatic treatment (Loperamide 4mg followed by 2 mg every 4 hours) with Probiotic yogurt (150 Grams) before breakfast and dinner twice a day for one week. Intervention 2: Control group: Symptomatic treatment Symptomatic treatment (Loperamide 4mg followed by 2 mg every 4 hours) with Regular yogurt (150 Grams) before breakfast and dinner twice a day for one week. Intervention 3: Control group: Symptomatic treatment Symptomatic treatment (Loperamide 4mg followed by 2 mg every 4 hours) with No intervention.
Primary outcome(s): Diarrhea frequency. Timepoint: Daily for one week after the start of the Intervention. Method of measurement: Patient’s report.;Diarrhea intensity. Timepoint: Daily for one week after the start of the Intervention. Method of measurement: Patient’s report.;Stool consistency. Timepoint: Daily for one week after the start of the Intervention. Method of measurement: Observation.
Study Design: Randomization: Randomized, Blinding: Not blinded, Placebo: Used, Assignment: Parallel, Purpose: Supportive, Randomization description: The study subjects were randomly allocated to yogurt + probiotic, yogurt, and control groups using Random Allocation Software (RAS) through random block sizes of 6 with an allocation ratio of 1:1. Random sequencing allocation was produced by the person who did not participate in the Sampling. The software produces sequences with 2 samples in each group. Containers were numbered from 1 to 90 according to the sequence generated.
Project description:Meta-analyses suggest that yogurt consumption reduces type 2 diabetes incidence in humans, but the molecular basis of these observations remains unknown. Here we show that dietary yogurt intake preserves whole-body glucose homeostasis and prevents hepatic insulin resistance and liver steatosis in a dietary mouse model of obesity-linked type 2 diabetes. Fecal microbiota transplantation studies reveal that these effects are partly linked to the gut microbiota. We further show that yogurt intake impacts the hepatic metabolome, notably maintaining the levels of branched chain hydroxy acids (BCHA) which correlate with improved metabolic parameters. These metabolites are generated upon milk fermentation and concentrated in yogurt. Remarkably, diet-induced obesity reduces plasma and tissue BCHA levels, and this is partly prevented by dietary yogurt intake. We further show that BCHA improve insulin action on glucose metabolism in liver and muscle cells, identifying BCHA as cell-autonomous metabolic regulators and potential mediators of yogurt's health effects.
Project description:Genome sequencing project for further understanding the molecular mechanisms of preventing non-alcoholic fatty liver disease Genome sequencing and assembly
Project description:The gut microbiota and fermentation-derived branched chain hydroxy acids mediate the health benefits of yogurt consumption in obese mice
Project description:With the aim of investigating the genome-wide postprandial effects of single servings ingestion of milk and yogurt on gene expression in the blood cells of human subjects and to identify the downstream physiological processes regulated by the differentially expressed genes we conducted a randomized, controlled, single blinded, crossover study on 6 healthy male individuals. 540g of milk or yogurt was ingested after an overnight fasting. Blood samples were collected before (0h) and 2h, 4h, 6h after the ingestion and the blood cell transcriptome was analyzed using a linear kinetic analysis.
