Project description:Panax ginseng C.A. Meyer is one of the most popular medicinal herbs. In order to research the genes that related to the flowering period of ginseng, and find out the antifungal proteins and transcription factors that combat various biotic and abiotic stress, a cDNA sample was prepared from the flowering period ginseng root of a five-year-old plant and sequenced using the Illumina sequencing platform. In this study, we produced nearly 40 million sequencing reads. These reads were assembled into 134,045 contigs using Trinity software (mean size: 282 bp). Based on a similarity search with known proteins, we identified 79,307 sequences with a cut-off E-value of 10-5. Assembled sequences were then annotated using gene ontology (GO) terms, clusters of orthologous group (COG) classifications and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways respectively.
Project description:Using maize cytolines (same nucleus but different cytoplasms), our research adds a new facet to the paradigm explaining gene expression changes in response to heat stress in an effort to maintain the homeostasis, linking the response to the genetic divergence of the nuclear and organellar genomes.
Project description:Next generation sequencing (NGS) was performed to identify genes changed in ginseng upon Botrytis cinerea △BcSpd1 treatment. The goal of the work is to find interesting genes involved in ginseng in response to fungi induction. The object is to reveal the molecular mechanism of ginseng defense induced by Botrytis cinerea △BcSpd1 .
Project description:Next-generation sequencing (NGS) was performed to identify genes changed in ginseng upon Colletotrichum panacicola infection. The goal of the work is to find interesting genes involved in ginseng in response to fungi induction. The object is to reveal the molecular mechanism of ginseng disease development caused by Colletotrichum panacicola.
Project description:In order to analyze the transcriptome of ginseng root during leaf-expansion period and discover the genes during development, a cDNA sample was prepared from the leaf-expansion period of ginseng root and sequenced using the Illumina sequencing platform.The transcriptomic sequencing technology was set up the first time for five years the transcription of the ginseng root in the leaf-expansion period.
Project description:Ginseng has been used for anti-stress agents, and its active ingredient, ginsenoside, has similar structure with estrogen. In this study, effects of red ginseng (RG) on gene expressions in immobilization (IMO) stressed mice brain was analyzed by system biology.
Project description:In order to research the ginseng leaf-stem gene expression profiles of and dig out its function genes in the leaf-expansion period, the transcriptomic sequencing technology was set up the first time for five years the transcription of the Panax ginseng leaf-stem in the leaf-expansion period.