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Effects and role(s) of the noncoding subgenomic RNA of red clover necrotic mosaic virus in virus infection

ABSTRACT: Effects and role(s) of the noncoding subgenomic RNA of red clover necrotic mosaic virus in virus infection

PROVIDER: PRJNA741487 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14915930_1.fastq.gz	Fastqsanger.gz
	SRR14915930_2.fastq.gz	Fastqsanger.gz
	SRR14915931_1.fastq.gz	Fastqsanger.gz
	SRR14915931_2.fastq.gz	Fastqsanger.gz
	SRR14915932_1.fastq.gz	Fastqsanger.gz

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Effects and role(s) of the noncoding subgenomic RNA of red clover necrotic mosaic virus in virus infection

Project description:In recent years, a new class of viral noncoding subgenomic (ncsg)RNA has been identified that is generated as a stable degradation product formed via an exonuclease-resistant (xr) RNA structure that blocks the progression of 5’-3’ exonuclease that degrades viral RNAs in infected cells. The xrRNA-derived ncsgRNAs have been implicated in virus pathogenicity, virus movement and transmission, and suppressing antiviral defense responses. Although ncsgRNAs of animal viruses have been studied extensively, research on the role of xrRNA-derived plant viral ncsgRNAs remain scarce. In this study, we assess the effects of the ncsgRNA of red clover necrotic mosaic virus (RCNMV), called SR1f, in infected plants. We demonstrate that the presence of SR1f leads to severe symptoms and increased viral RNA accumulation in Nicotiana benthamiana and Arabidopsis thaliana. We also provide evidence that suppression of RNA silencing is not the primary function of SR1f. Additionally, a comparative transcriptomic analysis in N. benthamiana infected with wildtype RCNMV and an SR1f-deficient mutant RCNMV reveal that wt RCNMV infection that produces SR1f has a greater and significant impact on cellular gene expression resulting in deregulation of several cellular pathways with upregulation of genes mainly involved in plant hormone signaling, plant-pathogen interaction, MAPK signaling, and several metabolic pathways and downregulation of genes involved mainly in photosynthesis.

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