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Collagen10a1-Cre;Rosa26-tdTomato Bone marrow colony forming units

ABSTRACT: Collagen10a1-Cre;Rosa26-tdTomato Bone marrow colony forming units

PROVIDER: PRJNA742567 | ENA |

REPOSITORIES: ENA

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Collagen10a1-Cre;Rosa26-tdTomato Bone marrow colony forming units

Project description:To assess if descendants of the hypertrophic chondrocytes behaved as skeletal stem and progenitor cells, we performed colony unit formation. We observed a near equal proportion of colonies derived from hypertrophic chondrocytes (tomato+) and derived from other sources (likely periosteum). We performed bulk RNA-sequencing on 3 colonies from each origin to compare the gene profiles and potential differentiation capacity or niche support.

2022-02-16 | GSE179174 | GEO

Prx1Cre;Rosa26-tdTomato single cell RNA-sequencing of muscle resident non-myogenic mesenchymal cells

Project description:In order to assess the descendants of lateral plate mesoderm within the muscle interstitium, we utilized Prrx1Cre;Rosa26-tdTomato P21 tdTomato+ FACS sorted muscle interstitial cells

2022-05-09 | GSE200234 | GEO

Human umbilical cord blood-derived endothelial colony forming cells under serum-free conditions

Project description:Derivation and expansion of human umbilical cord blood-derived endothelial colony forming cells under serum-free conditions - a transcriptome analysis. Endothelial colony forming cells (ECFCs) were isolated from term umbilical cord blood units. ECFCs were expanded under standard, fetal bovine serum (FBS) containing endothelial medium, or transferred to chemically defined endothelial media without FBS. Microarray expression profiling was applied to compare the transcriptome profiles in FBS-containing versus FBS-free culture.

2010-06-29 | GSE20504 | GEO

Collagen10-Cre;Rosa26-tdTomato e16.5 single cell RNA-sequencing

Project description:In order to assess the descendants of hypertrophic chondrocytes, we utilized Collagen10-Cre;Rosa26-tdTomato mouse total bone isolated at e16.5 by Collagenase II digestion after mechanical digestion and soft tissue removal. After sequencing and downstream analysis using Seurat, we observed clusters of cells with gene profiles matching classically defined chondrocytes, skeletal stem and progenitor cells (SSPCs), and osteoblasts. Trajectory analysis reveals that the SSPCs lie intermediate to the transition of chondrocyte to osteoblast. We conclude that hypertrophic chondrocytes dedifferentiate to this progenitor stage before further differentiation.

2022-02-16 | GSE190616 | GEO

Human umbilical cord blood-derived endothelial colony forming cells under serum-free conditions

2010-07-29 | E-GEOD-20504 | biostudies-arrayexpress

Collagen10-Cre;Rosa26-tdTomato e16.5 single cell RNA-sequencing

Project description:Collagen10-Cre;Rosa26-tdTomato e16.5 single cell RNA-sequencing

| PRJNA787704 | ENA

Collagen10-Cre;Rosa26-tdTomato bone marrow single cell RNA-sequencing

Project description:In order to assess the descendants of hypertrophic chondrocytes, we utilized Collagen10-Cre;Rosa26-tdTomato mouse bone marrow harvested at 2 months of age by centrifugation and light Collagenase II digestion. After sequencing and downstream analysis using Seurat, we observed clusters of cells with gene profiles matching classically defined skeletal stem and progenitor cells as well as CXCL12 abundant reticular (CAR) cells. These cells appear to be upstream of both osteoblasts and adipocytes. We conclude that hypertrophic chondrocytes dedifferentiate to this progentior stage before further differentiation.

2022-02-16 | GSE179148 | GEO

Collagen10-Cre;Rosa26-tdTomato bone marrow single cell RNA-sequencing

Project description:Collagen10-Cre;Rosa26-tdTomato bone marrow single cell RNA-sequencing

| PRJNA742416 | ENA

KSHV infection of Endothelial Colony Forming Cells

Project description:We performed high throughput RNA-sequencing on KSHV-infected blood and lymphatic Endothelial Colony-Forming Cells at 48hpi to identify differences in gene expression induced by KSHV in these two cell types.

2022-11-28 | GSE207589 | GEO

Identification and Characterization of Splenic Adherent Cells forming Densely-Packed Colony

Project description:As a first step for identifying hypothetical splenic stem cells for nonhematopoietic cells, densely-packed colony-forming cells were isolated from mouse spleen. Those which we designated as Splenic Adherent Colony-Forming Cells (SACCs) are positive for some of stem cell markers such as alkaline phosphatase and SSEA-1 antigen. We herewith determined global expression profiles of SACCs and control splenic adherent cells.

2019-06-25 | GSE117442 | GEO

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