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Taraxacum kok-saghyz

ABSTRACT: Taraxacum kok-saghyz Raw sequence reads

PROVIDER: PRJNA745038 | ENA |

REPOSITORIES: ENA

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Dataset's files

Source:

			Action	DRS
	SRR15094069_1.fastq.gz	Fastqsanger.gz
	SRR15094069_2.fastq.gz	Fastqsanger.gz
	SRR15094070_1.fastq.gz	Fastqsanger.gz
	SRR15094070_2.fastq.gz	Fastqsanger.gz
	SRR15094071_1.fastq.gz	Fastqsanger.gz

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Inflorescence Development and Floral Organogenesis in Taraxacum kok-saghyz.

Project description:Rubber dandelion (Taraxacum kok-saghyz Rodin; TK) has received attention for its natural rubber content as a strategic biomaterial, and a promising, sustainable, and renewable alternative to synthetic rubber from fossil carbon sources. Extensive research on the domestication and rubber content of TK has demonstrated TK's potential in industrial applications as a relevant natural rubber and latex-producing alternative crop. However, many aspects of its biology have been neglected in published studies. For example, floral development is still poorly characterized. TK inflorescences were studied by scanning electron microscopy. Nine stages of early inflorescence development are proposed, and floral micromorphology is detailed. Individual flower primordia development starts at the periphery and proceeds centripetally in the newly-formed inflorescence meristem. Floral organogenesis begins in the outermost flowers of the capitulum, with corolla ring and androecium formation. Following, pappus primordium-forming a ring around the base of the corolla tube-and gynoecium are observed. The transition from vegetative to inflorescence meristem was observed 21 days after germination. This description of inflorescence and flower development in TK sheds light on the complex process of flowering, pollination, and reproduction. This study will be useful for genetics, breeding, systematics, and development of agronomical practices for this new rubber-producing crop.

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Project description:Taraxacum kok-saghyz R.

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Population Genetics of the Rubber-Producing Russian Dandelion (Taraxacum kok-saghyz).

Project description:The Russian dandelion, Taraxacum kok-saghyz (TKS), is a perennial species native to Central Asia that produces high quality, natural rubber. Despite its potential to help maintain a stable worldwide rubber supply, little is known about genetic variation in this species. To facilitate future germplasm improvement efforts, we developed simple-sequence repeat (SSR) markers from available expressed-sequence tag (EST) data and used them to investigate patterns of population genetic diversity in this nascent crop species. We identified numerous SSRs (1,510 total) in 1,248 unigenes from a larger set of 6,960 unigenes (derived from 16,441 ESTs) and designed PCR primers targeting 767 of these loci. Screening of a subset of 192 of these primer pairs resulted in the identification of 48 pairs that appeared to produce single-locus polymorphisms. We then used the most reliable 17 of these primer pairs to genotype 176 individuals from 17 natural TKS populations. We observed an average of 4.8 alleles per locus with population-level expected heterozygosities ranging from 0.28 to 0.50. An average pairwise FST of 0.11 indicated moderate but statistically significant levels of genetic differentiation, though there was no clear geographic patterning to this differentiation. We also tested these 17 primer pairs in the widespread common dandelion, T. officinale, and a majority successfully produced apparently single-locus amplicons. This result demonstrates the potential utility of these markers for genetic analyses in other species in the genus.

| S-EPMC4703197 | biostudies-literature

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