Project description:To better understand the molecular bases of resin production, a major source of terpenes for industry, the transcriptome of adult Pinus elliottii var. elliottii (slash pine) trees under field commercial resinosis was obtained.
Project description:To address the increasing labor cost of resin tapping, more efficient methods for resin tapping need to be developed. This study aimed to evaluate the features of resinosis as affected by stimulant pastes in Pinus elliottii × P. caribaea, which is also one of the predominant resin-producing species hybrids in South China. The resin yields and resin compositions were assessed in 33 P. elliottii × P. caribaea F1 families, with the application of four kinds of chemical stimulants, potassium (K2SO4) paste, naphthalene acetic acid (NAA) paste, benzoic acid (BA) paste and 2-chloroethylphosphonic acid (CEPA) paste. Our results showed that all four pastes significantly increased the resin yield by at least 20% at each tapping, and 3- to fivefold increases were detected at the beginning of each year. The correlations between resin yield and growth at each tapping ranged from uncorrelated to moderately positively correlated, indicating that resin yield was mostly but not always determined by tree size. The concentration of each resin component did not change with the stimulant applications. In P. elliottii × P. caribaea, selecting a larger tree diameter at breast height and employing the chemical stimulants at the first several tapping rounds are efficient tapping procedures. Moreover, the K2SO4-based stimulant can be recommended considering its promoting effects on resin yield and the low cost of the chemicals required to produce it.
Project description:BackgroundResin-tapping forests of slash pine (Pinus elliottii) have been set up across Southern China owing to their high production and good resin quality, which has led to the rapid growth of the resin industry. In this study, we aimed to identify molecular markers associated with resin traits in pine trees, which may help develop marker-assisted selection (MAS).MethodsPeTPS-(-)Apin gene was cloned by double primers (external and internal). DnaSP V4.0 software was used to evaluate genetic diversity and linkage disequilibrium. SHEsis was used for haplotype analysis. SPSS was used for ANOVA and χ2 test. DnaSP v4.0 software was used to evaluate genetic diversity.ResultsThe full length PeTPS-(-)Apin gene was characterized and shown to have 4638 bp, coding for a 629-amino acid protein. A total of 72 single nucleotide polymorphism (SNP) loci were found. Three SNPs (CG615, AT641 and AG3859) were significantly correlated with α -pinene content, with a contribution rate > 10%. These SNPs were used to select P. elliottii with high α-pinene content, and a 118.0% realistic gain was obtained.ConclusionsThe PeTPS-(-)Apin gene is not uniquely decisive for selection of plus slash pines with stable production, high yield, and good quality, but it can be used as a reference for selection of other resin-producing pines and other resin components.