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Next-generation sequencing (NGS) analysis of GCA effects on BMSCs

ABSTRACT: Next-generation sequencing (NGS) analysis of GCA effects on BMSCs

PROVIDER: PRJNA749498 | ENA |

REPOSITORIES: ENA

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Next-generation sequencing (NGS) analysis of GCA effects on BMSCs

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Project description:Purpose: The goals of this study are to compare the effects of vehicle, PTH, and YKL-05-093 on gene expression by NGS-derived RNA-sequencing Methods: Ocy454 cells were grown at 37C for 14 days . Cells were then treated with vehicle, PTH (1 nM), or YKL-05-093 (0.5 uM) for 4 hours. Total RNA was isolated using Qiagen Rneasy columns, and submitted for NGS library generation and sequencing. All experiments performed in duplicate.

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