Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

NFI factors repress fetal hemoglobin in adult erythorid cells [CUT&RUN]

ABSTRACT: NFI factors repress fetal hemoglobin in adult erythorid cells [CUT&RUN]

PROVIDER: PRJNA749785 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

NFI factors repress fetal hemoglobin in adult erythorid cells

Project description:NFI factors repress fetal hemoglobin in adult erythorid cells

| PRJNA749793 | ENA

CUT&RUN of erythroblasts upon perturbation of gamma-hemoglobin repressors (part II)

Project description:Through high throughput compound screening, we've identified compounds that induce the expression of fetal hemoglobin. This study contains CUT&RUN data of a novel target, WIZ.

2024-06-19 | E-MTAB-13890 | biostudies-arrayexpress

NFI factors repress fetal hemoglobin in adult erythorid cells [CUT&RUN]

Project description:The mechanisms by which the fetal type b-globin-like genes HBG1 and HBG2 are silenced in adult erythroid precursor cells is a basic biology question in human development. Reversal of such mechanisms is beneficial for b hemoglobinopathies, such as sickle cell disease (SCD). A CRISPR-Cas9 genetic screen uncovered two members of the NFI transcription factor family – NFIA and NFIX – as novel HBG1/2 repressors. Both factors are expressed at elevated levels in adult erythroid cells, and their single or combined depletion revealed cooperativity in HBG1/2 regulation in cultured cells and human-to-mouse xenotransplant experiments, as well as in preventing sickling of SCD-derived erythroblasts. Genomic profiling, gene editing and in vitro binding assays demonstrated that the potent concerted activity of NFIA and NFIX factors is explained in part by their ability to activate expression of BCL11A, a known silencer of the HBG1/2 genes, and in part by repressing the HBG1/2 genes via two direct binding sites. NFI factors emerge as versatile regulators of the fetal-to-adult switch in b-globin production.

2022-03-23 | GSE180860 | GEO

NFI factors repress fetal hemoglobin in adult erythorid cells [ATAC-seq]

Project description:NFI factors repress fetal hemoglobin in adult erythorid cells [ATAC-seq]

| PRJNA749778 | ENA

NFI factors repress fetal hemoglobin in adult erythorid cells [RNA-seq]

Project description:NFI factors repress fetal hemoglobin in adult erythorid cells [RNA-seq]

| PRJNA749795 | ENA

RNA-seq and CUT&RUN of erythroblasts upon perturbation of gamma-hemoglobin repressors

Project description:Through high throughput compound screening, we've identified compounds that induce the expression of fetal hemoglobin. This study contains RNA-seq and CUT&RUN data of a novel target, WIZ.

2024-06-19 | GSE247096 | GEO

Bulk Cut&run and Cut&tag

Project description:Bulk Cut&run and Cut&tag

| PRJNA686390 | ENA

HIC2 represses BCL11A transcription to regulate hemoglobin switching during development [CUT&RUN]

Project description:HIC2 represses BCL11A transcription to regulate hemoglobin switching during development [CUT&RUN]

| PRJNA756674 | ENA

NFI factors repress fetal hemoglobin in adult erythorid cells [RNA-seq]

2022-03-23 | GSE180869 | GEO

NFI factors repress fetal hemoglobin in adult erythorid cells [ATAC-seq]

2022-03-23 | GSE180855 | GEO