Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The scRNA-seq of cells from Ren1c-/-;Myh11-CreERT2;tdTomato;Ren1c-YFP mice

ABSTRACT: The scRNA-seq of cells from Ren1c-/-;Myh11-CreERT2;tdTomato;Ren1c-YFP mice

PROVIDER: PRJNA749955 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR15257936.fastq.gz	Fastqsanger.gz

Items per page:

1 - 1 of 1

Similar Datasets

scRNA-seq of Achilles tendons from Prg4-CreERT2;R26-tdTomato ATP mice

Project description:scRNA-seq of Achilles tendons from Prg4-CreERT2;R26-tdTomato ATP mice

| PRJNA780190 | ENA

The scRNA-seq of cells from Ren1c-/-;Myh11-CreERT2;tdTomato;Ren1c-YFP mice

Project description:scRNA-seq was performed to investigate the differences between Reninnull cells and surrounding vascular smooth muscle cells

2021-12-22 | GSE180893 | GEO

RNA sequencing data of microglia isolated from brains of WT, Hexb-tdTomato, Hexb-CreERT2 and Hexb-KO mice. [Cre]

Project description:RNA sequencing of FACS sorted microglia isolated from brain tissue of WT, Hexb-tdTomato, Hexb-CreERT2 and Hexb-KO mice was performed to test for potential differences in gene expression caused by the knock-in of tdTomato and CreERT2 into the Hexb locus.

2020-05-25 | GSE148410 | GEO

RNA sequencing data of microglia isolated from brains of WT, Hexb-tdTomato, Hexb-CreERT2 and Hexb-KO mice. {Reporter]

2020-05-25 | GSE148412 | GEO

RNA sequencing data of microglia isolated from brains of WT, Hexb-tdTomato, Hexb-CreERT2 and Hexb-KO mice. [Hexb-KO]

2020-05-25 | GSE148411 | GEO

Generation and comparative analysis of an Itga8-CreERT2 mouse with preferential activity in vascular smooth muscle cells

Project description:All current smooth muscle cell (SMC) restricted Cre mice recombine floxed alleles in vascular and visceral SMCs. We generated a tamoxifen-inducible CreERT2 mouse, Itga8-CreERT2, and compared its activity to the widely used Myh11-CreERT2 mouse. Both CreERT2 mice showed similar activity in vascular SMCs; however, Itga8-CreERT2 displayed low activity in visceral SMC-containing tissues (e.g., intestine). Myh11-CreERT2 (but not Itga8-CreERT2) mice exhibited high levels of CreERT2 protein, tamoxifen-independent activity, and an altered transcriptome. Whereas Myh11-CreERT2-mediated knockout of Srf resulted in a lethal intestinal phenotype, loss of Srf with Itga8-CreERT2 (SrfItga8) revealed viable mice with attenuated vascular SMC contractile gene expression, but no evidence of intestinal pathology. Male and female SrfItga8 mice presented with vascular contractile incompetence; however, only male SrfItga8 mice showed systemic changes in blood pressure. These results establish the Itga8-CreERT2 mouse as an alternative to existing SMC Cre strains where gene loss may result in visceral myopathies that obfuscate accurate phenotyping in vascular SMCs.

2022-09-13 | GSE199244 | GEO

High throughput quantitative whole transcriptome analysis of CC10- B4+ and Krt5-CreERT2 - labeled distal lung cells

Project description:We used microfluidic single cell RNA-seq on adult isolated CC10-CreERT2 (negative) integrin beta4(pos) cells lung epithelial cells in order to determine the transcriptional profile of this putative progenitor population. CC10-CreERT2 / tdTomato (negative) integrin beta4(pos) cells were isolated by FACS, as were Krt5-CreERT2 / tdTomato (positive) cells. These cells were pooled and loaded onto the Fluidigm C1 device.

2014-11-17 | E-GEOD-61300 | biostudies-arrayexpress

Transcriptome profiling of tdTomato+ CGNPs from cerebellum of Math1-CreERT2 transgenic mice

Project description:Fluorescent cerebella were subsequently dissected at E15.5, E17.5, P0 (day of birth), P7 (postnatal day 7), P14 and P30; and tdTomato+ CGNPs were purified using fluorescence activated cell sorting (FACS). From these samples, we generated transcriptomes using RNA-seq

2022-09-25 | E-MTAB-7399 | biostudies-arrayexpress

Smartseq3 Single-cell RNA-seq analysis of acutely FACS-purified tdTomato+ astrocyte derived cells from aged injured mice in vivo

Project description:To investigate the mechanisms that underlie astrocyte dedifferentiation, we performed single cell RNA sequencing analysis of tdTomato+ astrocyte derived cells approx 1 year after stab-wound injury. 2-3 month old GFAP-CreERT2;p53flox/flox;LSL-tdTomato (p53Gfap-icKO) mice were subjected to intracortical endoxifen injection to enable deletion of p53 gene and simultaneous expression of tdTomato for fate-mapping specifically in astrocytes (GFAP+) in the context of a stab-wound injury. GFAP-CreERT2;p53wt/wt;LSL-tdTomato (p53Gfapwt/wt) mice were used as control whereby p53 remains fate-mapped tdTomato+ astrocytes remain p53 wildtype after endoxifen injection. Approx 10-12 months after injury, mice were sacrificed and the peri-wound region was dissected. Mice of the same genotypes were pooled (3 p53Gfap-icKO and 2 p53Gfapwt/wt) and digested using the Miltenyi Adult brain dissociation kit. Single cell suspension was FACS sorted for tdTomato+ cells into 384 well plates and then Smartseq3 libraries were generated.

2023-02-23 | GSE220479 | GEO

Comparative gene expression profiling analysis of scRNA-seq data for MyoD-tdTomato high expressing cultured muscle stem cells from MyoD KI mice

Project description:We previosuly generated MyoD-KI mice to distinguish the heterogenous MuSCs populations based on MYOD expression. Using the mice, we performed scRNA-seq to identify the unique gene expression profile in MyoD-tdTomato high expressing MuSCs.

2024-10-08 | GSE250228 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data